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牛蛙趾肌中的细胞内pH值、氢离子通量和氢离子渗透系数

Intracellular pH, H ion flux and H ion permeability coefficient in bullfrog toe muscle.

作者信息

Izutsu K T

出版信息

J Physiol. 1972 Feb;221(1):15-27. doi: 10.1113/jphysiol.1972.sp009735.

DOI:10.1113/jphysiol.1972.sp009735
PMID:4536964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1331316/
Abstract
  1. The dimethyloxazolidinedione (DMO) technique was used to estimate intracellular pH (pH(i)) in bullfrog toe muscles incubated in vitro. The control value of pH(i) was 7.16 + +/- 0.01 (S.D.).2. pH(i) was affected by changes in P(CO2) and external bicarbonate ion concentration (HCO(3) (-)). At a given P(CO2), decreasing the external [HCO(3) (-)] was more effective in lowering pH(i) than increasing the external [HCO(3) (-)] was in increasing pH(i).3. On the assumption that the changes in pH(i) were due to hydrogen ion [H(+)) movements across the membrane, a H(+) flux of 10(-13) mole/cm(2). sec was calculated. The corresponding H(+) permeability coefficient was 10(-3) cm/sec.4. The variability of the tissue CO(2) buffer value was examined.
摘要

  1. 采用二甲基恶唑烷二酮（DMO）技术对体外培养的牛蛙趾肌细胞内pH（pH(i)）进行评估。pH(i)的对照值为7.16±0.01（标准差）。

  2. pH(i)受二氧化碳分压（P(CO2)）和细胞外碳酸氢根离子浓度（HCO(3)(-)）变化的影响。在给定的P(CO2)下，降低细胞外[HCO(3)(-)]对降低pH(i)的作用比增加细胞外[HCO(3)(-)]对提高pH(i)的作用更显著。

  3. 假设pH(i)的变化是由于氢离子[H(+)]跨膜移动所致，计算得出H(+)通量为10(-13)摩尔/平方厘米·秒。相应的H(+)渗透系数为10(-3)厘米/秒。

  4. 对组织二氧化碳缓冲值的变异性进行了检测。

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本文引用的文献

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Phosphorescence in liquid scintillation counting of proteins.蛋白质液体闪烁计数中的磷光现象。
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