Civan M M, Shporer M
Biophys J. 1972 Apr;12(4):404-13. doi: 10.1016/S0006-3495(72)86092-2.
Whole striated muscles from the frog Rana esculenta were bathed in Ringer's solution enriched with H(2) (17)O; the muscle water was subsequently collected by vacuum distillation. The integrated intensity of the nuclear magnetic resonance (NMR) signal of (17)O in the muscle was measured to be approximately (3/4) of the signal observed in the distilled water. The phenomenon may arise either from immobilization of a population of the water molecules which may be a very small fraction or as much as (1/4) of the total, or may reflect tumbling of (1/3) of the water molecules in a compartment containing an anisotropic medium. Such an effect was demonstrated for H(2) (17)O using the model system of sodium linoleate in water.
将食用蛙的完整横纹肌置于富含H₂¹⁷O的任氏液中；随后通过真空蒸馏收集肌肉中的水分。经测量，肌肉中¹⁷O的核磁共振（NMR）信号的积分强度约为蒸馏水中观测到信号的(3/4)。该现象可能源于一部分水分子的固定化，这部分水分子可能占总数的极小部分或多达(1/4)，也可能反映了在含有各向异性介质的隔室中(1/3)的水分子的翻滚。使用水中亚油酸钠的模型系统对H₂¹⁷O证实了这种效应。
