Neville M C
Science. 1972 Apr 21;176(4032):302-3. doi: 10.1126/science.176.4032.302.
In isolated frog muscle that has been incubated for 7 days at 0 degrees C invitro K(+) and Na(+) remain at normal concentrations. Amino acids are accumulated against a concentration gradient at this temperature; for example, glycine accumulates in muscle to a concentration ten times that in the external solution. The amount of cycloleucine accumulated is greater at 0 degrees C than at 25 degrees C. These findings, which are difficult to explain on the basis of metabolically linked active transport, are Consiststent with the view that solute accumulation by cell is the result of adsorption on spesific sites.
在0℃体外培养7天的离体青蛙肌肉中，钾离子（K⁺）和钠离子（Na⁺）浓度保持正常。在此温度下，氨基酸会逆浓度梯度积累；例如，肌肉中的甘氨酸积累到外部溶液中浓度的十倍。在0℃时积累的环亮氨酸量比在25℃时更多。这些发现很难基于代谢相关的主动转运来解释，这与细胞溶质积累是吸附在特定位点的结果这一观点一致。
