Phillips S M, Wegmann T G
J Exp Med. 1973 Feb 1;137(2):291-300. doi: 10.1084/jem.137.2.291.
Previous work has indicated that tetraparental mice, chimeric since the eight-cell stage because of embryo fusion using histoincompatible strain combinations, possess autospecific immune cells and blocking antibodies. Although this phenomenon has been demonstrated in vitro, it may have relevance to the self-tolerance shown by these mice in vivo. The experiments described here indicate that spleen cells from tetraparental mice can block mixed lymphocyte reactions between the two parental cell types, but not between unrelated strains. Furthermore, this suppressive ability is not affected by an otherwise effective treatment of the tetraparental spleen cells with anti-theta antibody and complement. The in vitro experimental system elaborated here should help to characterize the cell type responsible for the suppression.
先前的研究表明，四亲代小鼠由于使用组织不相容的品系组合进行胚胎融合，自八细胞期起即为嵌合体，其拥有自身特异性免疫细胞和封闭抗体。尽管这一现象已在体外得到证实，但它可能与这些小鼠在体内表现出的自身耐受性有关。此处所描述的实验表明，四亲代小鼠的脾细胞能够阻断两种亲代细胞类型之间的混合淋巴细胞反应，但不能阻断无关品系之间的反应。此外，用抗θ抗体和补体对四亲代脾细胞进行的有效处理，并不影响其这种抑制能力。这里所构建的体外实验系统应有助于鉴定负责这种抑制作用的细胞类型。
