Kappler J W, Hoffmann M
J Exp Med. 1973 Jun 1;137(6):1325-37. doi: 10.1084/jem.137.6.1325.
The kinetics of the in vivo response to SRBC was studied in mouse spleen at both the B cell and T cell levels. The B cell response was assayed by following the appearance of antibody-secreting cells in the spleen using the hemolytic plaque assay. The T cell response was monitored by following the increase in or "priming" of helper activity in the spleen using a quantitative in vitro assay. The role of cellular proliferation in both responses was established with the inhibitor of mitosis, vinblastine. The results show that, although the development of T cell activity precedes that of anti-SRBC PFC by as much as 1 day, T cells lag at least 1 day behind B cells in the onset of cellular proliferation. The evidence suggests either that the helper T cell which proliferates in response to SRBC does so after helping in the initiation of the primary B cell response or that the proliferative T cell response and the initiation of the primary B cell response involve two different subpopulations of T cells.
在小鼠脾脏中，从B细胞和T细胞水平研究了对绵羊红细胞（SRBC）体内反应的动力学。通过溶血空斑试验追踪脾脏中抗体分泌细胞的出现来测定B细胞反应。使用定量体外试验，通过追踪脾脏中辅助活性的增加或“启动”来监测T细胞反应。用有丝分裂抑制剂长春花碱确定细胞增殖在两种反应中的作用。结果表明，尽管T细胞活性的发展比抗SRBC空斑形成细胞（PFC）的发展早多达1天，但在细胞增殖开始时，T细胞比B细胞至少滞后1天。证据表明，要么是对SRBC作出反应而增殖的辅助性T细胞在帮助启动原发性B细胞反应之后才增殖，要么是增殖性T细胞反应和原发性B细胞反应的启动涉及T细胞的两个不同亚群。
