通过淋巴细胞转化试验研究抗体产生。I. CBA小鼠对绵羊红细胞的脾脏反应。
Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes.
作者信息
Wortis H H, Taylor R B, Dresser D W
出版信息
Immunology. 1966 Dec;11(6):603-16.
Abstract
By means of a modified plaque assay the numbers of cells producing 19S antibody (direct PFC) and 7S antibody (developed PFC) have been studied separately. Significant and somewhat surprising differences in the responses of the two kinds of antibody-producing cell have been noted. It is clear that the route of injection of the antigen is of importance in determining the response in the spleen. The response of both types of PFC was found to be biphasic. The total increase in spleen cell number after immunization was not readily accounted for by the increase in specific PFC.
摘要
通过改良的空斑测定法，已分别研究了产生19S抗体的细胞（直接空斑形成细胞）和产生7S抗体的细胞（发育空斑形成细胞）的数量。已注意到两种产生抗体细胞的反应存在显著且有点令人惊讶的差异。显然，抗原的注射途径在决定脾脏反应方面很重要。发现两种类型的空斑形成细胞的反应都是双相的。免疫后脾细胞数量的总体增加不能简单地用特异性空斑形成细胞的增加来解释。
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