Stender S
Atherosclerosis. 1979 Feb;32(2):129-39. doi: 10.1016/0021-9150(79)90078-9.
The arterial walls of 3 cholesterol-fed rabbits were exposed for 3--4 hours in vivo to homologous cholesterol-labelled plasma with a 20-fold higher [3H/14C] ratio in esterified cholesterol (EC) than in free cholesterol (FC). The [3H/14C] ratio in total cholesterol (TC) in the thoracic aorta was 0.6--0.9 times the ratio prevailing in TC in plasma. This corresponds to a transfer from plasma into the arterial wall of relatively more FC, with the low ratio, than EC with the higher ratio. The [3H/14C] ratio in FC in the arterial wall was 1.4--2.0 times the ratio in FC in plasma. This corresponds to hydrolysis of some of the entered EC, adding cholesterol with the high ratio to FC with the lower ratio. The [3H/14C] ratio in EC in the arterial wall was 0.1--1.1 times the ratio in EC in plasma. This corresponds to no esterification in the arterial wall of entered FC. The same method applied to normolipidemic cockerels gave similar results. In a kinetic model the arterial influx from plasma of FC and EC combined (lipoprotein influx) corresponded in both groups to 20--90 nl plasma per cm2 arterial surface per hour with subsequent hydrolysis of 10--50% of the entered EC. The arterial influx of FC without EC (influx by exchange) accounted for 10--25% of the total influx of cholesterol in the rabbits and 40--70% in the cockerels.
给3只喂食胆固醇的兔子的动脉壁在体内暴露于同源的胆固醇标记血浆3 - 4小时，该血浆中酯化胆固醇（EC）的[³H/¹⁴C]比值比游离胆固醇（FC）高20倍。胸主动脉中总胆固醇（TC）的[³H/¹⁴C]比值是血浆中TC比值的0.6 - 0.9倍。这意味着与具有较高比值的EC相比，相对更多具有较低比值的FC从血浆转移到了动脉壁中。动脉壁中FC的[³H/¹⁴C]比值是血浆中FC比值的1.4 - 2.0倍。这相当于一些进入的EC发生了水解，将具有较高比值的胆固醇添加到了具有较低比值的FC中。动脉壁中EC的[³H/¹⁴C]比值是血浆中EC比值的0.1 - 1.1倍。这表明进入的FC在动脉壁中没有发生酯化。将相同方法应用于血脂正常的公鸡也得到了类似结果。在一个动力学模型中，两组中FC和EC组合的血浆动脉内流（脂蛋白内流）相当于每平方厘米动脉表面每小时20 - 90纳升血浆，随后进入的EC有10 - 50%发生水解。无EC的FC动脉内流（通过交换的内流）占兔子胆固醇总内流的10 - 25%，占公鸡的40 - 70%。
