Ramseier H
J Exp Med. 1974 Sep 1;140(3):603-18. doi: 10.1084/jem.140.3.603.
In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates reproduced faithfully those found with the corresponding cells. Large amounts of receptors were released by untreated spleen cells and by spleen cells treated with a rabbit antimouse immunoglobulin serum and complement, smaller amounts were released by bone marrow cells, and native thymus cells released none. Spleen cells from nude mice and spleen cells from normal mice treated with anti-theta serum and complement showed no release of receptors. It was concluded that receptors active in the PAR test were of T-cell origin. Release of T-cell receptors was found to be discontinuous and proceeded in waves. The amount of released receptors depended on the number of cells cultivated. Release occurred at 37 degrees C but not at 4 degrees C. Interaction with antigen, however, was temperature-independent. In contrast to T-cell receptors, a release of H-2 antigens could not be detected with the culture conditions employed.
对小鼠脾细胞进行体外培养，导致同种异体抗原受体的自发释放。这通过无细胞培养上清液识别同种异体抗原的能力得以揭示，该能力在PAR试验中进行测定。定性地说，用这些上清液获得的识别反应如实地重现了用相应细胞所发现的反应。未处理的脾细胞以及用兔抗小鼠免疫球蛋白血清和补体处理的脾细胞释放大量受体，骨髓细胞释放少量受体，而天然胸腺细胞不释放受体。裸鼠的脾细胞以及用抗θ血清和补体处理的正常小鼠的脾细胞未显示受体释放。得出的结论是，PAR试验中具有活性的受体源自T细胞。发现T细胞受体的释放是不连续的且呈波浪式进行。释放的受体数量取决于培养的细胞数量。释放在37℃时发生，但在4℃时不发生。然而，与抗原的相互作用与温度无关。与T细胞受体相反，在所采用的培养条件下未检测到H-2抗原的释放。
