胸腺和脾脏淋巴细胞的细胞表面半乳糖基转移酶与凝集素凝集作用
Cell surface galactosyltransferase and lectin agglutination of thymus and spleen lymphocytes.
作者信息
Lamont J T, Perrotto J L, Weiser M M, Isselbacher K J
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3726-30. doi: 10.1073/pnas.71.9.3726.
Lectin agglutination and cell surface galactosyltransferase (EC 2.4.1.67; 1-O-alpha-D-galactosyl-myo-inositol:raffinose galactosyltransferase) enzyme activity have been studied with thymus and spleen lymphocytes of neonatal rats. Thymus lymphocytes were more agglutinable by concanavalin A than by wheat germ agglutinin, whereas spleen lymphocytes were more agglutinable by wheat germ agglutinin than by concanavalin A. Thymus lymphocytes, but not spleen lymphocytes, of neonatal rats could be blast transformed by concanavalin A. Cell surface galactosyltransferase activity was present on both types of lymphocytes, but was greatly increased on thymus cells after blast transformation. The differences in lectin agglutination suggest a difference in the surface membranes of thymus and spleen lymphocytes. The increase in cell surface galactosyltransferase activity with blast transformation of thymus lymphocytes may be related to the exteriorization of the Golgi apparatus into the plasma membrane.
对新生大鼠的胸腺和脾淋巴细胞进行了凝集素凝集及细胞表面半乳糖基转移酶（EC 2.4.1.67；1-O-α-D-半乳糖基-肌醇：棉子糖半乳糖基转移酶）活性的研究。胸腺淋巴细胞对伴刀豆球蛋白A的凝集性高于对麦胚凝集素的凝集性，而脾淋巴细胞对麦胚凝集素的凝集性高于对伴刀豆球蛋白A的凝集性。新生大鼠的胸腺淋巴细胞而非脾淋巴细胞可被伴刀豆球蛋白A转化为母细胞。两种类型的淋巴细胞均存在细胞表面半乳糖基转移酶活性，但在母细胞转化后，胸腺细胞的该活性大幅增加。凝集素凝集的差异表明胸腺和脾淋巴细胞的表面膜存在差异。胸腺淋巴细胞母细胞转化后细胞表面半乳糖基转移酶活性的增加可能与高尔基体向质膜的外排有关。
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