Epstein L, Cline M J
Clin Exp Immunol. 1974 Apr;16(4):553-63.
The present study employs a new technique for the study of human T-cell effector function in patients with chronic lymphocytic leukaemia: mitogen-stimulated interferon. Cultures of macrophages, T cell-enriched lymphocytes, or macrophages and lymphocytes combined were prepared from the blood of fourteen normal donors and five patients with chronic lymphocytic leukaemia. The effects of the mitogens, phytohaemagglutinin and pokeweed, on interferon production and lymphocyte transformation were studied and the following observations made: (a) T-cell effector and proliferative functions were depressed as evidenced by the absence of interferon and proliferative response to PHA and PWM at 3 days ; (b) Three out of five patients showed no interferon or proliferative response at 6 days, thus indicating a B lymphocyte abnormality as well; (c) macrophages from both normal and leukaemic subjects augmented mitogen-stimulated lymphocyte interferon production and lymphocyte transformation. However, the addition of normal allogeneic macrophages to cultures of lymphocytes prepared from the patients did not restore the proliferative and interferon responses to normal levels.
本研究采用一种新技术来研究慢性淋巴细胞白血病患者的人类T细胞效应功能：丝裂原刺激的干扰素。从14名正常供体和5名慢性淋巴细胞白血病患者的血液中制备巨噬细胞、富含T细胞的淋巴细胞或巨噬细胞与淋巴细胞的组合培养物。研究了丝裂原（植物血凝素和商陆）对干扰素产生和淋巴细胞转化的影响，并得出以下观察结果：（a）T细胞效应和增殖功能受到抑制，这在3天时缺乏干扰素以及对PHA和PWM的增殖反应中得到证明；（b）5名患者中有3名在6天时没有干扰素或增殖反应，因此也表明存在B淋巴细胞异常；（c）正常和白血病患者的巨噬细胞均增强了丝裂原刺激的淋巴细胞干扰素产生和淋巴细胞转化。然而，将正常异体巨噬细胞添加到患者制备的淋巴细胞培养物中并不能将增殖和干扰素反应恢复到正常水平。
