小鼠淋巴细胞的玫瑰花结形成。I. 通过间接免疫荧光法证明T细胞与绵羊红细胞的结合。
Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes.
作者信息
Gyöngyössy M I, Playfair J H
出版信息
Clin Exp Immunol. 1974 Oct;18(2):169-76.
Abstract
A method is described for indirect immunofluorescent staining of mouse T cells forming rosettes, involving fixation of rosettes by glutaraldehyde and staining with a rabbit anti-mouse brain serum absorbed so as to be specific for T cells. Using this technique, it was shown that about 44% of spleen cells forming rosettes with sheep erythrocytes both in non-immunized and immunized mice, are T cells. The number of T rosette-forming cells increased about ten-fold after immunization with sheep erythrocytes, but only when serum antibody was detectable.
摘要
本文描述了一种对形成玫瑰花结的小鼠T细胞进行间接免疫荧光染色的方法，该方法包括用戊二醛固定玫瑰花结，并用经吸收处理以使其对T细胞具有特异性的兔抗小鼠脑血清进行染色。使用该技术表明，在未免疫和免疫的小鼠中，与绵羊红细胞形成玫瑰花结的脾细胞中约44%是T细胞。在用绵羊红细胞免疫后，T玫瑰花结形成细胞的数量增加了约10倍，但仅在可检测到血清抗体时才会增加。
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引用本文的文献
1
Rosette formation by mouse lymphocytes. III. Receptors for immunoglobulin on normal and activated T cells.小鼠淋巴细胞的玫瑰花结形成。III. 正常和活化T细胞上免疫球蛋白的受体
Clin Exp Immunol. 1974 Oct;18(2):187-92.
2
Rosette formation by mouse lymphocytes. II. T-cell specificities in a CRL subpopulation.小鼠淋巴细胞的玫瑰花结形成。II. CRL亚群中的T细胞特异性。
Clin Exp Immunol. 1974 Oct;18(2):177-86.
3
4
5
Complement-induced mixed aggregation of T and B lymphocytes and myeloid cells and the effect of adjuvant.补体诱导的T淋巴细胞、B淋巴细胞和髓系细胞混合聚集及佐剂的作用。
Clin Exp Immunol. 1975 Jul;21(1):115-20.
本文引用的文献
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Strain differences in the immune response of mice. I. The neonatal response to sheep red cells.小鼠免疫反应中的品系差异。I. 对绵羊红细胞的新生反应。
Immunology. 1968 Jul;15(1):35-50.
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In vivo specific antigen recognition by rosette forming cells.体内玫瑰花结形成细胞对特异性抗原的识别。
Nature. 1970 Sep 19;227(5264):1251-2. doi: 10.1038/2271251a0.
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Theta isoantigen as a marker of thymus-derived lymphocytes in mice.θ同种抗原作为小鼠胸腺来源淋巴细胞的标志物。
Nature. 1969 Oct 25;224(5217):378-9. doi: 10.1038/224378a0.
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T and B rosette-forming cells.T 细胞和 B 细胞玫瑰花结形成细胞。
Eur J Immunol. 1971 Dec;1(6):501-3. doi: 10.1002/eji.1830010622.
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Selective priming of T cells by chemically altered cell antigens.通过化学改变的细胞抗原来选择性启动T细胞。
J Exp Med. 1972 Sep 1;136(3):656-61. doi: 10.1084/jem.136.3.656.
6
Studies on antigen-binding cells. II. Relationship to antigen-sensitive cells.抗原结合细胞的研究。II. 与抗原敏感细胞的关系。
Cell Immunol. 1970 Oct;1(4):386-403. doi: 10.1016/0008-8749(70)90016-x.
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Studies on antigen-binding cells. I. The origin of reactive cells.抗原结合细胞的研究。I. 反应性细胞的起源。
Cell Immunol. 1970 Oct;1(4):372-85. doi: 10.1016/0008-8749(70)90015-8.
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Antigen-binding cells in normal mouse thymus.正常小鼠胸腺中的抗原结合细胞。
Science. 1970 Dec 4;170(3962):1102-4. doi: 10.1126/science.170.3962.1102.
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Anti-theta antibodies for detecting thymus-dependent lymphocytes in the immune response of mice to SRBC.用于检测小鼠对绵羊红细胞免疫反应中胸腺依赖性淋巴细胞的抗θ抗体。
Nature. 1970 Jun 27;226(5252):1254-6. doi: 10.1038/2261254a0.
10
Indirect immunofluorescence of mouse thymus-derived cells using heterologous anti-brain serum.使用异种抗脑血清对小鼠胸腺来源细胞进行间接免疫荧光检测。
Cell Immunol. 1973 Apr;7(1):118-23. doi: 10.1016/0008-8749(73)90187-1.
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