小鼠淋巴细胞的玫瑰花结形成。I. 通过间接免疫荧光法证明T细胞与绵羊红细胞的结合。
Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes.
作者信息
Gyöngyössy M I, Playfair J H
出版信息
Clin Exp Immunol. 1974 Oct;18(2):169-76.
Abstract
A method is described for indirect immunofluorescent staining of mouse T cells forming rosettes, involving fixation of rosettes by glutaraldehyde and staining with a rabbit anti-mouse brain serum absorbed so as to be specific for T cells. Using this technique, it was shown that about 44% of spleen cells forming rosettes with sheep erythrocytes both in non-immunized and immunized mice, are T cells. The number of T rosette-forming cells increased about ten-fold after immunization with sheep erythrocytes, but only when serum antibody was detectable.
摘要
本文描述了一种对形成玫瑰花结的小鼠T细胞进行间接免疫荧光染色的方法，该方法包括用戊二醛固定玫瑰花结，并用经吸收处理以使其对T细胞具有特异性的兔抗小鼠脑血清进行染色。使用该技术表明，在未免疫和免疫的小鼠中，与绵羊红细胞形成玫瑰花结的脾细胞中约44%是T细胞。在用绵羊红细胞免疫后，T玫瑰花结形成细胞的数量增加了约10倍，但仅在可检测到血清抗体时才会增加。
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引用本文的文献
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Rosette formation by mouse lymphocytes. III. Receptors for immunoglobulin on normal and activated T cells.
Clin Exp Immunol. 1974 Oct;18(2):187-92.
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Clin Exp Immunol. 1975 Mar;19(3):485-97.
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