Hall W
Immunology. 1973 Aug;25(2):158-96.
From 7 to 35 days after CBA mice were primed with SRBC their spleens were removed and anti-SRBC rosettes were formed. The rosettes were purified from other spleen cells by velocity sedimentation at 4° and rosette-enriched, rosette-depleted and various control populations were injected into lethally irradiated CBA recipients. These were challenged with SRBC and their spleens analysed for direct (IgM) and enhanced (IgG) PFC 7 days later. Removal of RFC depleted the primed spleen cells of their capacity adoptively to transfer an immune response. This depletion was antigen-specific. Purified rosettes alone prepared 7–8 days after priming transferred significant (relative to controls) immune reactivity to the irradiated recipients. Both B and T RFC were present at this stage and the response was dependent upon cell collaboration between these two populations. Later in the primary response (35 days) purified rosettes transferred negligible immune reactivity. But these RFC (90 per cent B) collaborated with rosette-depleted cells to restore full reactivity. B memory lymphocytes (AFCP) form rosettes from 7 to 35 days after immunization but T memory cells only do so for a limited stage during the peak of the primary response. The majority of T memory cells probably never form rosettes in this system. It is suggested that most T RFC may be cells mediating delayed hypersensitivity or are `passive' rosettes.
用绵羊红细胞（SRBC）对CBA小鼠进行初次免疫后7至35天，取出它们的脾脏并形成抗SRBC玫瑰花结。通过在4℃下进行速度沉降从其他脾细胞中纯化玫瑰花结，并将富含玫瑰花结、去除玫瑰花结的细胞群及各种对照细胞群注射到经致死剂量照射的CBA受体小鼠体内。给这些受体小鼠注射SRBC进行攻击，7天后分析它们脾脏中的直接（IgM）和增强（IgG）的空斑形成细胞（PFC）。去除玫瑰花结形成细胞（RFC）会使致敏脾细胞丧失过继转移免疫反应的能力。这种去除是抗原特异性的。初次免疫后7 - 8天制备的单独纯化玫瑰花结能将显著的（相对于对照）免疫反应性转移给受照射的受体小鼠。在这个阶段，B和T RFC都存在，且反应依赖于这两个细胞群之间的协作。在初次反应后期（35天），纯化玫瑰花结转移的免疫反应性可忽略不计。但这些RFC（90%为B细胞）与去除玫瑰花结的细胞协作可恢复完全的反应性。B记忆淋巴细胞（AFCP）在免疫后7至35天形成玫瑰花结，但T记忆细胞仅在初次反应高峰期的有限阶段形成玫瑰花结。在这个系统中，大多数T记忆细胞可能从未形成过玫瑰花结。有人提出，大多数T RFC可能是介导迟发型超敏反应的细胞或为“被动”玫瑰花结。
