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5-溴脱氧尿苷在“无病毒”人细胞（补体结合抗原-免疫荧光-白细胞）中激活爱泼斯坦-巴尔病毒

Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes).

作者信息

Gerber P

出版信息

Proc Natl Acad Sci U S A. 1972 Jan;69(1):83-5. doi: 10.1073/pnas.69.1.83.

DOI:10.1073/pnas.69.1.83
PMID:4550512
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427549/
Abstract

Treatment of three human lymphoid cell lines, free of detectable Epstein-Barr virus, with 5-bromodeoxyuridine resulted in activation of virus synthesis in up to 8% of the cells. The induction of infectious virus could be demonstrated by cocultivation experiments with peripheral leukocytes. These studies demonstrated that the entire viral genome may persist in at least a portion of the lymphoid cells.

摘要

用5-溴脱氧尿苷处理三种未检测到爱泼斯坦-巴尔病毒的人类淋巴细胞系，导致高达8%的细胞中病毒合成被激活。通过与外周血白细胞共培养实验可证明有感染性病毒的诱导。这些研究表明整个病毒基因组可能至少在一部分淋巴细胞中持续存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930b/427549/5d7129f046a6/pnas00127-0090-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930b/427549/763839a509df/pnas00127-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930b/427549/5d7129f046a6/pnas00127-0090-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930b/427549/763839a509df/pnas00127-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930b/427549/5d7129f046a6/pnas00127-0090-b.jpg

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Activation of Epstein-Barr virus by 5-bromodeoxyuridine in "virus-free" human cells (complement-fixing antigen-immunofluorescence-leukocytes).5-溴脱氧尿苷在“无病毒”人细胞（补体结合抗原-免疫荧光-白细胞）中激活爱泼斯坦-巴尔病毒
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