Cox C S, Goldberg L J
Appl Microbiol. 1972 Jan;23(1):1-3. doi: 10.1128/am.23.1.1-3.1972.
The aerosol survival in air was determined for Pasteurella tularensis live vaccine strain (LVS) as a function of relative humidity (RH). Three different preparations of bacteria were used: (i) liquid suspension of P. tularensis LVS in spent culture medium; (ii) powders of P. tularensis LVS freeze-dried in spent culture fluid; (iii) P. tularensis LVS freeze-dried in spent culture fluid and then reconstituted with distilled water and disseminated as a liquid suspension. Preparation (i) gave greatest survival at high RH and lowest survival at intermediate RH. Preparation (ii), in contrast, gave greatest survival at low RH and minimum survival at 81% RH. Preparation (iii) was the same as preparation (i), i.e., the process of freeze-drying and reconstituting with distilled water before aerosol formation had little or no effect upon aerosol survival as a function of RH. Hence, control of aerosol survival appears to be through the water content of P. tularensis LVS at the moment of aerosol generation rather than the water content of the bacteria in the aerosol phase.
测定了土拉弗朗西斯菌活疫苗株（LVS）在空气中的气溶胶存活率与相对湿度（RH）的关系。使用了三种不同的细菌制剂：（i）土拉弗朗西斯菌LVS在废弃培养基中的液体悬浮液；（ii）在废弃培养液中冻干的土拉弗朗西斯菌LVS粉末；（iii）在废弃培养液中冻干的土拉弗朗西斯菌LVS，然后用蒸馏水复溶并作为液体悬浮液散播。制剂（i）在高相对湿度下存活率最高，在中等相对湿度下存活率最低。相比之下，制剂（ii）在低相对湿度下存活率最高，在81%相对湿度下存活率最低。制剂（iii）与制剂（i）相同，即在形成气溶胶之前进行冻干并用蒸馏水复溶的过程，对作为相对湿度函数的气溶胶存活率几乎没有影响。因此，气溶胶存活率的控制似乎是通过气溶胶产生瞬间土拉弗朗西斯菌LVS的含水量，而不是气溶胶相中细菌的含水量。
