一种来自兔网织红细胞的核糖体解离因子（氟化物-大肠杆菌核糖体-起始因子）。
A ribosome dissociation factor from rabbit reticulocytes (fluoride-E. coli ribosomes-initiation factors).
作者信息
Lubsen N H, Davis B D
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):353-7. doi: 10.1073/pnas.69.2.353.
Abstract
A ribosome dissociation factor has been detected in an extract of ribosomal particles from rabbit reticulocytes. This factor dissociates free ribosomes from reticulocytes and also from Escherichia coli; it does not dissociate ribosomes complexed with peptidyl-tRNA and mRNA. The reaction appears to be stoichiometric rather than catalytic; it reaches completion in one minute at 37 degrees C, but is very slow at 0 degrees C, and it is antagonized and reversed by Mg(++). Reticulocyte dissociation factor thus closely resembles that from E. coli. However, the activity has been found primarily associated with the native large subunits rather than the small subunits in lysates.
摘要
在兔网织红细胞核糖体颗粒提取物中检测到一种核糖体解离因子。该因子可使网织红细胞以及大肠杆菌中的游离核糖体解离；但不会使与肽基 - tRNA和mRNA结合的核糖体解离。该反应似乎是化学计量的而非催化性的；在37℃下1分钟内反应完成，但在0℃时非常缓慢，并且Mg(++)可拮抗并逆转该反应。因此，网织红细胞解离因子与大肠杆菌的解离因子非常相似。然而，已发现该活性主要与裂解物中的天然大亚基相关，而非小亚基。
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A ribosome dissociation factor from rabbit reticulocytes (fluoride-E. coli ribosomes-initiation factors).一种来自兔网织红细胞的核糖体解离因子（氟化物-大肠杆菌核糖体-起始因子）。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The size and hydration of rabbit-reticulocyte ribosomes.兔网织红细胞核糖体的大小与水合作用
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Evidence for a naF-resistant association between mRNA and ribosomes in rabbit reticulocytes.家兔网织红细胞中mRNA与核糖体之间存在对新霉素不敏感关联的证据。
Biochim Biophys Acta. 1967 Mar 29;138(1):186-8. doi: 10.1016/0005-2787(67)90599-0.
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Sedimentation studies on the interaction of ribosomal subunits from liver.肝脏核糖体亚基相互作用的沉降研究。
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Ribosomal subunit exchange in the cytoplasm of a eukaryote.真核生物细胞质中的核糖体亚基交换。
Nature. 1969 Jun 7;222(5197):950-3. doi: 10.1038/222950a0.
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Initiation of protein synthesis in a rabbit reticulocyte lysate system.兔网织红细胞裂解液系统中蛋白质合成的起始
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Subunit recycling during translation in a reticulocyte cell-free system.
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