双链RNA对细胞蛋白质合成的抑制作用：一种起始因子的失活
Inhibition of cellular protein synthesis by double-stranded RNA: inactivation of an initiation factor.
作者信息
Kaempfer R, Kaufman J
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1222-6. doi: 10.1073/pnas.70.4.1222.
Abstract
Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA is caused by the inactivation of IF-3, an initiation factor required for the recycling of ribosomes and for their binding to messenger RNA. The evidence for this is that (i) the inhibition can be overcome by addition of exogenous If-3; (ii) double-stranded RNA inactivates stoichiometric amounts of IF-3; (iii) double-stranded RNA forms a complex with IF-3; and (iv) double-stranded DNA, which lacks inhibitory activity, also binds to IF-3, but with a much lower affinity than double-stranded RNA. It is concluded that double-stranded RNA inhibits cellular protein synthesis by tightly complexing with IF-3. It is suggested that IF-3 normally recognizes a double-stranded region in messenger RNA.
摘要
双链RNA对兔网织红细胞裂解物中蛋白质合成的抑制作用是由IF-3失活引起的，IF-3是核糖体循环及其与信使RNA结合所需的起始因子。支持这一观点的证据如下：（i）添加外源性IF-3可克服这种抑制作用；（ii）双链RNA使化学计量的IF-3失活；（iii）双链RNA与IF-3形成复合物；（iv）缺乏抑制活性的双链DNA也与IF-3结合，但亲和力远低于双链RNA。得出的结论是，双链RNA通过与IF-3紧密结合来抑制细胞蛋白质合成。有人提出，IF-3通常识别信使RNA中的双链区域。
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