抗血清对基因转移的抑制作用及性菌毛血清型的鉴定
Inhibition of gene transfer by antiserum and identification of serotypes of sex pili.
作者信息
Harden V, Meynell E
出版信息
J Bacteriol. 1972 Mar;109(3):1067-74. doi: 10.1128/jb.109.3.1067-1074.1972.
Abstract
Gene transfer by conjugation due to F or R (drug resistance) factors is inhibited by antibody to the sex pili. Serological analysis is able to distinguish between the sex pili determined by closely related sex factors, and the specificity of inhibition of transfer agrees with that previously determined by direct electron microscopical observation of antibody bound to the sex pili (10). Inhibition of transfer can therefore be applied to the identification of wild-type R factors with repressed sex factors that determine too few pili to be examined directly. It can also be used to differentiate the activities of two unrelated sex factors in the same donor bacterium.
摘要
由F因子或R（耐药性）因子介导的接合作用所导致的基因转移会受到抗性菌毛抗体的抑制。血清学分析能够区分由密切相关的性因子所决定的性菌毛，并且转移抑制的特异性与先前通过直接电子显微镜观察抗体结合到性菌毛上所确定的特异性一致（10）。因此，转移抑制可用于鉴定带有受抑制性因子的野生型R因子，这些受抑制性因子所决定的菌毛数量过少而无法直接检测。它还可用于区分同一供体细菌中两个不相关的性因子的活性。
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