萘啶酸对结合细菌的作用机制。
Mechanism of action of nalidixic acid on conjugating bacteria.
作者信息
Bouck N, Adelberg E A
出版信息
J Bacteriol. 1970 Jun;102(3):688-701. doi: 10.1128/jb.102.3.688-701.1970.
Abstract
When nalidixic acid, a specific and effective inhibitor of cellular deoxyribonucleic acid synthesis, is added to conjugating bacteria at any time during mating, it stops genetic transfer provided the donor bacterium is sensitive to the drug. When this inhibition is released by the removal of the nalidixic acid, transfer does not resume at the point on the chromosome where it was stopped, but begins again at the transfer origin. Curves relating the effects of various low doses of nalidixic acid to the frequency of recombination reveal that several "hits" are necessary to inhibit recombination for early markers. The number of required "hits" decreases as the distance of the marker from the transfer origin increases. Transfer between drug-resistant cells may also be inhibited by nalidixic acid. The effect of high drug doses on matings between resistant cells is similar to that of low drug doses on matings with a sensitive male.
摘要
萘啶酸是一种细胞脱氧核糖核酸合成的特异性有效抑制剂，在交配过程中的任何时候将其添加到正在进行接合的细菌中，只要供体细菌对该药物敏感，它就会停止基因转移。当通过去除萘啶酸解除这种抑制作用时，转移不会在染色体上停止的位置恢复，而是在转移起点重新开始。将各种低剂量萘啶酸的作用与重组频率相关的曲线表明，对于早期标记物，抑制重组需要几个“命中”。所需“命中”的数量随着标记物与转移起点距离的增加而减少。萘啶酸也可能抑制耐药细胞之间的转移。高剂量药物对耐药细胞之间交配的影响与低剂量药物对与敏感雄性细胞交配的影响相似。
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