Stevenson K E, Vaughn R H, Crisan E V
J Bacteriol. 1972 Mar;109(3):1295-7. doi: 10.1128/jb.109.3.1295-1297.1972.
A triple fixation method using a sequential application of 15 or 30% formaldehyde, 6% glutaraldehyde, and 1% osmium tetroxide resulted in excellent fixation of mature spores of Clostridium botulinum.
一种采用依次施加15%或30%甲醛、6%戊二醛和1%四氧化锇的三重固定方法,对肉毒梭菌的成熟孢子实现了极佳的固定效果。