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用选定抗原性的人工切片进行免疫荧光评估。

Evaluation of immunofluorescence with artificial sections of selected antigenicity.

作者信息

Brandtzaeg P

出版信息

Immunology. 1972 Jan;22(1):177-83.

PMID:4552229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1408216/
Abstract

An inert matrix of polymerized normal rabbit serum was given defined antigenicity by allowing selected human proteins to diffuse into small pieces of the gel. After fixation and paraffin embedding, serial sections with uniform distribution of reactive antigen were obtained. Such sections are recommended for determination of specificity and optimal working titre of fluorescent antibodies; as substrate for titration of serum antibodies; and as standards for quantification of tissue antigens.

摘要

通过让选定的人类蛋白质扩散到小块凝胶中，使聚合的正常兔血清惰性基质具有特定的抗原性。固定并石蜡包埋后，获得了反应性抗原分布均匀的连续切片。推荐使用此类切片来测定荧光抗体的特异性和最佳工作效价；作为血清抗体滴定的底物；以及作为组织抗原定量的标准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd9b/1408216/a51379738aa0/immunology00348-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd9b/1408216/a51379738aa0/immunology00348-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd9b/1408216/a51379738aa0/immunology00348-0181-a.jpg

相似文献

1
Evaluation of immunofluorescence with artificial sections of selected antigenicity.用选定抗原性的人工切片进行免疫荧光评估。
Immunology. 1972 Jan;22(1):177-83.
2
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本文引用的文献

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SOLUBLE ANTIGEN FLUORESCENT-ANTIBODY TECHNIQUE.可溶性抗原荧光抗体技术
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AUTOIMMUNITY AND PERIODONTAL DISEASE.自身免疫与牙周病
Odontol Tidskr. 1965 Jun 30;73:281-393.
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IMMUNOFLUORESCENCE APPLIED TO PROTEIN SOLUTIONS.免疫荧光技术应用于蛋白质溶液
用于免疫细胞化学和原位杂交的载玻片模型。
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Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma.具有不同倍性的大肠癌，与上皮组织和血浆中的分泌成分、免疫球蛋白A及癌胚抗原相关。
Br J Cancer. 1982 Jun;45(6):921-34. doi: 10.1038/bjc.1982.145.
6
Evaluation of nine different fixatives. 2. Preservation of IgG, IgA and secretory component in an artificial immunohistochemical test substrate.九种不同固定剂的评估。2. 在人工免疫组织化学检测底物中IgG、IgA和分泌成分的保存
Histochemistry. 1984;81(3):213-9. doi: 10.1007/BF00495630.
7
Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocytochemistry of lymphomas.淋巴瘤免疫细胞化学组织制备方法及配对免疫荧光染色的评估
Histochem J. 1983 Jul;15(7):655-89. doi: 10.1007/BF01002987.
8
Sensitivity and efficiency of four immunohistochemical methods as defined by staining of artificial sections.通过人工切片染色定义的四种免疫组织化学方法的敏感性和效率。
Histochemistry. 1984;81(4):313-9. doi: 10.1007/BF00514325.
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A step-wedge standard for the quantification of immunoperoxidase techniques.免疫过氧化物酶技术定量的阶梯楔形标准。
Histochem J. 1982 Jul;14(4):609-20. doi: 10.1007/BF01011893.
10
Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique.免疫球蛋白成分的黏膜和腺体分布。采用冷乙醇固定技术的免疫组织化学。
Immunology. 1974 Jun;26(6):1101-14.
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4
The fluorescent antibody technique applied to titration and identification of antigens in solutions or antisera.
Proc Soc Exp Biol Med. 1963 Jun;113:394-7. doi: 10.3181/00379727-113-28375.
5
Preparation of a semipermanent mounting medium for fluorescent antibody studies.用于荧光抗体研究的半永久性封固介质的制备。
Virology. 1960 Oct;12:316-7. doi: 10.1016/0042-6822(60)90205-1.
6
Quantification of immunofluorescence on individual erythrocytes coated with varying amounts of antigen.对涂有不同量抗原的单个红细胞上的免疫荧光进行定量分析。
Immunology. 1970 Jul;19(1):151-6.
7
Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.异硫氰酸荧光素与抗体的结合。II. 一种可重复的方法。
Immunology. 1970 Jun;18(6):875-81.
8
The application of microphotometry to the indirect immunofluorescent antibody technique using the human gastric parietal cell antigen-antibody system.显微光度测定法在使用人胃壁细胞抗原 - 抗体系统的间接免疫荧光抗体技术中的应用。
Clin Exp Immunol. 1969 May;4(5):607-17.
9
Microphotometry in immunofluorescence.免疫荧光中的显微光度测定法。
Clin Exp Immunol. 1969 Jun;4(6):697-705.
10
Standardization in immunofluorescence.免疫荧光标准化
Clin Exp Immunol. 1968 Jul;3(6):465-76.