Mouton R P, Stoop J W, Ballieux R E, Mul N A
Clin Exp Immunol. 1970 Aug;7(2):201-10.
An indirect immuno-fluorescent method is described for the detection of group and type specific antibodies against pneumococci. The localization of these antibodies in the various immunoglobulin classes can be established by this method. Difficulties may be encountered, due to masking of sites on the antigen by antibodies, present in excess in a particular class of immunoglobulin. Relatively high titres of group specific and type specific antibodies against pneumococcus types, 3, 6, 9, 14 and 24 could be demonstrated in colostrum IgA. Occasionally these antibodies were also found in serum IgA. Group specific antibodies were detected in low titres in IgA of saliva and nasal secretions. The findings are discussed in relation to the concept of the secretory IgA system.
本文描述了一种间接免疫荧光法，用于检测抗肺炎球菌的群特异性和型特异性抗体。通过该方法可以确定这些抗体在各种免疫球蛋白类别中的定位。由于特定免疫球蛋白类别中过量存在的抗体掩盖了抗原上的位点，可能会遇到困难。初乳IgA中可检测到针对肺炎球菌3型、6型、9型、14型和24型的相对高滴度的群特异性和型特异性抗体。偶尔在血清IgA中也发现这些抗体。在唾液和鼻分泌物的IgA中检测到低滴度的群特异性抗体。结合分泌型IgA系统的概念对这些发现进行了讨论。
