Winkelhake J L, Elcombe B M, Hodach A
Cancer Res. 1979 Aug;39(8):3058-64.
Antibodies to formalin-fixed, syngeneic melanoma cells were prepared in mice, purified by immunoaffinity chromatography, and tested for binding activity to viable melanoma cells. The radiolabeled antibodies detected congruent to 9 X 10(6) melanoma antigenic sites/cell. The calculated average association constant (Ka) for the antibody population was 7 to 10 X 10(7) M-1. The antibody was shown to block the binding of melanocyte-stimulating hormone in competitive cell surface binding studies. Results are discussed conceptually in terms of the potentially important role that the humoral immune response may play in the phenomenon of tumor progression.
针对福尔马林固定的同基因黑色素瘤细胞的抗体在小鼠体内制备，通过免疫亲和层析进行纯化，并检测其与活的黑色素瘤细胞的结合活性。放射性标记的抗体检测到每个细胞约有9×10⁶个黑色素瘤抗原位点。计算得出抗体群体的平均缔合常数（Ka）为7至10×10⁷ M⁻¹。在竞争性细胞表面结合研究中，该抗体可阻断促黑素细胞激素的结合。从体液免疫反应在肿瘤进展现象中可能发挥的潜在重要作用的角度，对结果进行了概念性讨论。
