在S生物体提取物中，铁氧化还原蛋白依赖的乙醛向乙酸盐和氢气的转化。
Ferredoxin-dependent conversion of acetaldehyde to acetate and H 2 in extracts of S organism.
作者信息
Reddy C A, Bryant M P, Wolin M J
出版信息
J Bacteriol. 1972 Apr;110(1):133-8. doi: 10.1128/jb.110.1.133-138.1972.
Abstract
S organism, an anaerobic gram-negative rod, which is one of two bacterial species isolated from the culture known as "Methanobacillus omelianskii," ferments ethanol to acetate and H(2). The present study shows that extracts of this organism contain ferredoxin and produce acetate from acetaldehyde via aldehyde: ferredoxin oxidoreductase activity. Electrons generated in the reaction are given off as H(2) by a previously demonstrated ferredoxin-linked hydrogenase system. Extracts were shown to contain good phosphotransacetylase and acetokinase activities, but no mechanism of adenosine triphosphate generation during acetaldehyde conversion to acetate could be detected. No evidence could be obtained for coenzyme A (CoASH) or phosphate requirement or for formation of acetyl CoA or acetyl phosphate.
摘要
S菌是一种厌氧革兰氏阴性杆菌，是从名为“奥氏甲烷杆菌”的培养物中分离出的两种细菌之一，它可将乙醇发酵为乙酸盐和H₂。本研究表明，该菌的提取物含有铁氧化还原蛋白，并通过醛：铁氧化还原蛋白氧化还原酶活性从乙醛生成乙酸盐。反应中产生的电子通过先前证明的与铁氧化还原蛋白相关的氢化酶系统以H₂的形式释放。提取物显示具有良好的磷酸转乙酰酶和乙酰激酶活性，但在乙醛转化为乙酸盐的过程中未检测到三磷酸腺苷生成机制。未获得关于辅酶A（CoASH）或磷酸盐需求或乙酰辅酶A或乙酰磷酸盐形成的证据。
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本文引用的文献
1
An electron transport factor from Clostridium pasteurianum.来自巴氏梭菌的一种电子传递因子。
Biochem Biophys Res Commun. 1962 Jun 4;7:448-52. doi: 10.1016/0006-291x(62)90333-9.
2
SOME PROPERTIES OF METHANOBACTERIUM OMELIANSKII FERREDOXIN.奥氏甲烷杆菌铁氧化还原蛋白的一些特性。
J Bacteriol. 1964 Sep;88(3):806-7. doi: 10.1128/jb.88.3.806-807.1964.
3
ANAEROBIC FORMATE OXIDATION: A FERREDOXIN-DEPENDENT REACTION.厌氧甲酸氧化：一种依赖铁氧化还原蛋白的反应。
Science. 1964 Apr 17;144(3616):297-8. doi: 10.1126/science.144.3616.297.
4
A comparison of clostridial ferredoxins.梭菌型铁氧化还原蛋白的比较
Proc Natl Acad Sci U S A. 1963 Mar 15;49(3):345-53. doi: 10.1073/pnas.49.3.345.
5
Enzymatic phosphorylation of acetate.乙酸的酶促磷酸化作用。
J Biol Chem. 1954 Dec;211(2):737-56.
6
The oxidation of acetaldehyde to acetyl coenzyme A.乙醛氧化为乙酰辅酶A。
J Biol Chem. 1953 Jun;202(2):873-90.
7
Hydrogenase measurement with photochemically reduced methyl viologen.用光化学还原甲基紫精进行氢化酶测量。
J Bacteriol. 1969 Apr;98(1):51-5. doi: 10.1128/jb.98.1.51-55.1969.
8
Methanobacillus omelianskii, a symbiotic association of two species of bacteria.奥氏甲烷杆菌，一种由两种细菌组成的共生联合体。
Arch Mikrobiol. 1967;59(1):20-31. doi: 10.1007/BF00406313.
9
Characteristics of S organism isolated from Methanobacillus omelianskii.从奥氏甲烷杆菌中分离出的S菌的特性。
J Bacteriol. 1972 Feb;109(2):539-45. doi: 10.1128/jb.109.2.539-545.1972.
10
Ethanol utilization by Steptococcus faecalis.
Arch Biochem Biophys. 1969 Aug;133(1):137-43. doi: 10.1016/0003-9861(69)90497-4.
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