从“奥氏甲烷杆菌”中分离出的S菌提取物中，铁氧化还原蛋白和烟酰胺腺嘌呤二核苷酸依赖型的乙醇和甲酸盐产氢过程。
Ferredoxin- and nicotinamide adenine dinucleotide-dependent H 2 production from ethanol and formate in extracts of S organism isolated from "Methanobacillus omelianskii".
作者信息
Reddy C A, Bryant M P, Wolin M J
出版信息
J Bacteriol. 1972 Apr;110(1):126-32. doi: 10.1128/jb.110.1.126-132.1972.
Abstract
S organism ferments ethanol to acetate and H(2) but grows poorly on ethanol unless the partial pressure of H(2) is kept low, as when it is grown in combination with an H(2)-utilizing methanogenic bacterium. The present study shows that S organism contains an alcohol dehydrogenase and a formate dehydrogenase, both of which require nicotinamide adenine dinucleotide (NAD) for activity. Hydrogen is evolved from NADH generated by these activities via a ferredoxin-dependent oxidation of NADH to NAD and H(2). NADH:NADP oxido-reductase activity was also demonstrated. The relationship of these activities to the growth of S organism is discussed.
摘要
S 菌体能将乙醇发酵为乙酸盐和氢气，但在乙醇上生长较差，除非氢气的分压保持较低，例如当它与利用氢气的产甲烷细菌共同培养时。本研究表明，S 菌体含有一种乙醇脱氢酶和一种甲酸脱氢酶，这两种酶的活性均需要烟酰胺腺嘌呤二核苷酸（NAD）。通过铁氧化还原蛋白将 NADH 氧化为 NAD 和氢气，这些活性产生的 NADH 会释放出氢气。还证实了 NADH:NADP 氧化还原酶活性。讨论了这些活性与 S 菌体生长的关系。
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