多肽链延伸因子结构基因的染色体定位。
Chromosomal localization of the structural genes of the polypeptide chain elongation factors.
作者信息
Gordon J, Baron L S, Schweiger M
出版信息
J Bacteriol. 1972 Apr;110(1):306-12. doi: 10.1128/jb.110.1.306-312.1972.
Abstract
A survey of the polypeptide chain elongation factors in potentially sexually compatible genera was carried out. Factors from Escherichia coli and Proteus mirabilis were found to be clearly distinguishable by immunochemical and electrophoretic techniques. Mapping of the structural genes of these factors was undertaken by a study of the gene products in genetically defined E. coli-P. mirabilis hybrid diploid strains. It was found that the EF G factor mapped within 5 min of the streptomycin resistance locus, but the EF Ts factor did not map in this region.
摘要
对潜在性兼容属中的多肽链延伸因子进行了一项调查。通过免疫化学和电泳技术发现，来自大肠杆菌和奇异变形杆菌的因子有明显区别。通过对基因定义的大肠杆菌-奇异变形杆菌杂交二倍体菌株中的基因产物进行研究，对这些因子的结构基因进行了定位。结果发现，EF G因子定位于链霉素抗性位点5分钟范围内，但EF Ts因子不在该区域定位。
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