Dubnau E, Pifko S, Sloma A, Cabane K, Smith I
Mol Gen Genet. 1976 Aug 10;147(1):1-12. doi: 10.1007/BF00337929.
Four temperature sensitive mutants of B. subtilis were isolated by localized mutagenesis in the major ribosomal gene cluster, and charcterized genetically and biochemically. Three are mutations which cause temperature sensitivity in the elongation factor Ef-G, and one which has a similar effect on the elongation factor Ef-Tu. They map in a cluster near strA, with the temperature sensitive mutations in Ef-G mapping between the strA gene and the temperature sensitive mutation in Ef-TU.
通过在主要核糖体基因簇中进行定位诱变，分离出了枯草芽孢杆菌的四个温度敏感突变体，并对其进行了遗传和生化特性分析。其中三个突变导致延伸因子Ef-G出现温度敏感性，另一个对延伸因子Ef-Tu有类似影响。它们定位于靠近strA的一个簇中，Ef-G中的温度敏感突变位于strA基因和Ef-TU中的温度敏感突变之间。
