二氨基庚二酸添加酶活性低的大肠杆菌K-12温度敏感突变体。
Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.
作者信息
Lugtenberg E J, v Schijndel-van Dam A
出版信息
J Bacteriol. 1972 Apr;110(1):41-6. doi: 10.1128/jb.110.1.41-46.1972.
Abstract
Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 42 C. NaCl had no influence on the Dpm adding enzyme activity in vitro. The growth rate of most temperature-resistant revertants was decreased, but their Dpm adding enzyme activity remained very low. Two revertants had a rather normal growth rate. Their Dpm adding enzyme activity was significantly increased, but much lower than in the wild type. The influence of growth rate on the viability of the mutants is discussed.
摘要
发现五个温度敏感型裂解突变体在体外具有非常低的二氨基庚二酸（Dpm）添加酶活性。在42℃时，胞壁质合成受到损害，尿苷-5'-二磷酸-N-乙酰胞壁酰-L-丙氨酸-d-谷氨酸（UDP-MurNAc-二肽）积累。在氯化钠存在的情况下，这些突变体可以在42℃生长。氯化钠对体外Dpm添加酶活性没有影响。大多数温度抗性回复突变体的生长速率降低，但其Dpm添加酶活性仍然非常低。两个回复突变体具有相当正常的生长速率。它们的Dpm添加酶活性显著增加，但远低于野生型。讨论了生长速率对突变体活力的影响。
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8
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J Bacteriol. 1972 Jan;109(1):326-35. doi: 10.1128/jb.109.1.326-335.1972.
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