Coutard M, Osborne-Pellegrin M J
Cell Tissue Res. 1979 Apr 12;197(3):531-8. doi: 10.1007/BF00233575.
The localization in the mouse brain of corticosterone, the natural glucocorticoid in the mouse, and cortexolone, reported to be a glucocorticoid antagonist, was studied by autoradiography 30 min after in vivo administration of the tritiated compounds. After 3H-corticosterone (3HB) injection, radioactivity was preferentially concentrated in cell nuclei of several structures within the limbic system, and in nuclei of certain neurones of the cerebral cortex and medullar oblongata. This nuclear concentration was abolished after injection of 3H-corticosterone with an excess of unlabelled corticosterone. After 3H-cortexolone (3HS) injection, a diffuse radioactivity was observed throughout the brain. However, a higher concentration of grains was present in the ventral nucleus arcuatus and in the infundibulum. When excess unlabelled cortexolone was administered with 3H-cortexolone this preferential accumulation of grains was abolished. The accumulation of 3H-cortexolone in the medial basal hypothalamic region suggests that cortexolone concentrates preferentially in dexamethasone (DM) target regions, and in addition the autoradiographic results show that the cortexolone-receptor complex does not accumulate in the cell nucleus.
在体内给予氚标记化合物30分钟后，通过放射自显影术研究了小鼠体内天然糖皮质激素皮质酮以及据报道为糖皮质激素拮抗剂的皮质素olone在小鼠脑中的定位。注射3H-皮质酮（3HB）后，放射性优先集中在边缘系统内几个结构的细胞核以及大脑皮层和延髓某些神经元的细胞核中。注射过量未标记皮质酮的3H-皮质酮后，这种核内聚集消失。注射3H-皮质素olone（3HS）后，在整个大脑中观察到弥漫性放射性。然而，在腹侧弓状核和漏斗中存在较高浓度的银粒。当与3H-皮质素olone一起给予过量未标记的皮质素olone时，这种银粒的优先积累消失。3H-皮质素olone在内侧基底下丘脑区域的积累表明，皮质素olone优先集中在地塞米松（DM）靶区域，此外放射自显影结果表明皮质素olone-受体复合物不积累在细胞核中。
