来自不同动物物种的气管外植体分泌的糖蛋白的组成。

Composition of glycoproteins secreted by tracheal explants from various animal species.

Kaizu T, Lyons S A, Cross C E, Jennings M D, Last J A

California Primate Research Center, University of California, Davis 95616.

Comp Biochem Physiol B. 1979;62(3):195-200. doi: 10.1016/0305-0491(79)90199-8.

Abstract

The acidic and neutral glycoproteins secreted by cultured tracheal explants from pigs, sheep, rats, mice, monkeys, guinea pigs, dogs and chickens were purified and fractionated by column chromatography on DEAE-cellulose and by electrophoresis on cellulose acetate. 2. The ratios of acidic to neutral mucus glycoproteins were compared for the above animals with that of mucus glycoproteins secreted by cultured human bronchi. 3. The observed ratios of acidic to neutral glycoproteins ranged from 4.0 (mouse) to 7.2 (chicken and pig) from cultured tracheae; secreted human bronchial mucus had a ratio of 2.7. 4. The ratio of acidic to neutral glycoproteins secreted by tracheal explants varied with duration of incubation of the trachea in culture.

摘要

对猪、羊、大鼠、小鼠、猴子、豚鼠、狗和鸡的培养气管外植体分泌的酸性和中性糖蛋白进行纯化，并通过DEAE - 纤维素柱色谱法和醋酸纤维素电泳法进行分级分离。2. 将上述动物的酸性与中性黏液糖蛋白的比例与培养的人支气管分泌的黏液糖蛋白的比例进行比较。3. 培养气管中观察到的酸性与中性糖蛋白的比例范围为4.0（小鼠）至7.2（鸡和猪）；分泌的人支气管黏液的比例为2.7。4. 气管外植体分泌的酸性与中性糖蛋白的比例随气管在培养中的孵育时间而变化。