利用P1转导互补对大肠杆菌性因子F介导的转移进行遗传分析。
Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.
作者信息
Willetts N, Achtman M
出版信息
J Bacteriol. 1972 Jun;110(3):843-51. doi: 10.1128/jb.110.3.843-851.1972.
Abstract
P1 transduction has been used to perform a complementation analysis of a series of transfer-deficient mutants of Flac. The results define ten cistrons and are consistent with the results of a conjugational analysis presented in an accompanying report. Both sets of results are summarized here. Between them, they define eleven cistrons, traA through traK, necessary for conjugational deoxyribonucleic acid (DNA) transfer. Mutants in traI and traD and some in traG still make F-pili, although traD mutants are resistant to f2 phage; their products may be involved in conjugational DNA metabolism. Other mutants in traG and all mutants in the remaining eight cistrons do not make F-pili. One of these, traJ, may be a control cistron, and the others may specify a biosynthetic pathway responsible for synthesis and modification of the F-pilin subunit protein and its assembly into the F-pilus.
摘要
P1转导已用于对Flac一系列转移缺陷型突变体进行互补分析。结果确定了十个顺反子，并且与随附报告中进行的接合分析结果一致。此处总结了两组结果。它们共同确定了11个顺反子，即traA至traK，这些是接合脱氧核糖核酸（DNA）转移所必需的。traI和traD中的突变体以及traG中的一些突变体仍然产生F菌毛，尽管traD突变体对f2噬菌体具有抗性；它们的产物可能参与接合DNA代谢。traG中的其他突变体以及其余八个顺反子中的所有突变体均不产生F菌毛。其中一个，traJ，可能是一个控制顺反子，而其他顺反子可能指定了负责F菌毛蛋白亚基的合成、修饰及其组装成F菌毛的生物合成途径。
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