Ippen-Ihler K, Achtman M, Willetts N
J Bacteriol. 1972 Jun;110(3):857-63. doi: 10.1128/jb.110.3.857-863.1972.
A series of Hfr deletion mutants was isolated. These mutants contain deletions which extend from a lambda prophage into an Flac which is integrated into the gal operon. Transfer-deficient deletion mutants were found to fall into four different phenotypic groups when tested for male- and female-specific phage resistance. Conjugational and transductional complementation tests with Flac point mutants deficient in transfer (tra(-)) were performed, and the order of 11 tra cistrons was determined. The tra genes are all located between an F gene for the inhibition of female-specific phages and the transposed lac operon originally carried by the Flac. The order of genes in the Hfr studied was established to be: proC... phi(II) (R)... traJ traA traE traK traB traC traF traH traG traD traI...lac...attlambda...bio.
分离出一系列高频重组（Hfr）缺失突变体。这些突变体包含从一个λ原噬菌体延伸到整合到半乳糖操纵子中的Flac的缺失。当对雄性和雌性特异性噬菌体抗性进行测试时，发现转移缺陷型缺失突变体分为四个不同的表型组。用转移缺陷（tra(-)）的Flac点突变体进行了接合和转导互补试验，并确定了11个tra顺反子的顺序。tra基因都位于抑制雌性特异性噬菌体的F基因和最初由Flac携带的转座乳糖操纵子之间。所研究的Hfr中的基因顺序确定为：proC...phi(II)(R)...traJ traA traE traK traB traC traF traH traG traD traI...lac...attlambda...bio。
