大肠杆菌中弗氏志贺氏菌转移缺陷型突变体的接合互补分析。
Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.
作者信息
Achtman M, Willetts N, Clark A J
出版信息
J Bacteriol. 1972 Jun;110(3):831-42. doi: 10.1128/jb.110.3.831-842.1972.
Abstract
A series of 102 transfer-deficient (tra(-)) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F'/F') heterozygote cells in transient populations, and for measuring complementation between different Flac tra(-) mutants. These techniques were used to define nine tra cistrons and to assign most of the tra(-) mutations to one or other of these.
摘要
弗氏志贺菌（Flac）的102个转移缺陷型（tra(-)）突变体（其中84个先前已有描述）被分类为携带移码、琥珀色、赭石色、UGA和非抑制性突变。已开发出一些技术，用于将F'菌株的培养物用作交配中的高效受体，用于测量瞬时群体中（F'/F'）杂合细胞的数量，以及用于测量不同Flac tra(-)突变体之间的互补作用。这些技术被用于定义9个tra顺反子，并将大多数tra(-)突变分配到其中一个或另一个顺反子。
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Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.通过分离和鉴定转移缺陷型突变体，对大肠杆菌中由F因子决定的接合转移进行遗传分析。
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