通过Genetron萃取，随后进行连续流超速离心制备伯纳特柯克斯体的纯化悬浮液。
Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.
作者信息
Dubois D R, Cutchins E C, Berman S, Lowenthal J P, Timchak R L
出版信息
Appl Microbiol. 1972 May;23(5):841-5. doi: 10.1128/am.23.5.841-845.1972.
Abstract
A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.
摘要
描述了一种通过Genetron提取，随后进行连续流密度梯度超速离心来制备纯化的伯氏考克斯氏体悬浮液的方法。在53%至65%（w/w）蔗糖之间的区域发现了伯氏考克斯氏体亨泽林菌株的两个相。基于化学分析，发现Genetron区带立克次氏体悬浮液与目前用于生产人用Q热疫苗的醚提取法制备的立克次氏体悬浮液一样纯净。
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Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.通过Genetron萃取，随后进行连续流超速离心制备伯纳特柯克斯体的纯化悬浮液。
Appl Microbiol. 1972 May;23(5):841-5. doi: 10.1128/am.23.5.841-845.1972.
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