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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Slattery J T, Levy G

Clin Biochem. 1979 Jun;12(3):100-3. doi: 10.1016/s0009-9120(79)80075-2.

A liquid chromatographic method has been developed for the simultaneous determination of the anti-inflammatory agent naproxen and its metabolite, 6-0-desmethyl-naproxen, in human plasma or serum. 2. After addition of p-chlorowarfarin as the internal stardard, plasma is acidified and extracted with either chloroform (for assay of naproxen only) or ethyl acetate (for assay of naproxen and desmethyl-naproxen), the organic solvent is evaporated, the residue is dissolved in acetonitrile and injected onto a reverse phase column coupled with an ultra-violet absorbance detector. 3. Drug and metabolite can be detected readily in concentrations above 2 micrograms per ml in 0.5 ml samples. Salicylic acid and its metabolites and a number of other durgs were found not to interfere with the assay.

已开发出一种液相色谱法，用于同时测定人血浆或血清中的抗炎药物萘普生及其代谢物6-0-去甲基萘普生。2. 添加对氯华法林作为内标后，将血浆酸化，并用氯仿（仅用于测定萘普生）或乙酸乙酯（用于测定萘普生和去甲基萘普生）萃取，蒸发有机溶剂，将残留物溶解于乙腈中，然后注入与紫外吸收检测器相连的反相柱。3. 在0.5毫升样品中，浓度高于每毫升2微克时，药物和代谢物能够很容易地被检测到。发现水杨酸及其代谢物以及许多其他药物不会干扰该测定。

Slattery J T, Levy G

Clin Biochem. 1979 Jun;12(3):100-3. doi: 10.1016/s0009-9120(79)80075-2.

A liquid chromatographic method has been developed for the simultaneous determination of the anti-inflammatory agent naproxen and its metabolite, 6-0-desmethyl-naproxen, in human plasma or serum. 2. After addition of p-chlorowarfarin as the internal stardard, plasma is acidified and extracted with either chloroform (for assay of naproxen only) or ethyl acetate (for assay of naproxen and desmethyl-naproxen), the organic solvent is evaporated, the residue is dissolved in acetonitrile and injected onto a reverse phase column coupled with an ultra-violet absorbance detector. 3. Drug and metabolite can be detected readily in concentrations above 2 micrograms per ml in 0.5 ml samples. Salicylic acid and its metabolites and a number of other durgs were found not to interfere with the assay.

已开发出一种液相色谱法，用于同时测定人血浆或血清中的抗炎药物萘普生及其代谢物6-0-去甲基萘普生。2. 添加对氯华法林作为内标后，将血浆酸化，并用氯仿（仅用于测定萘普生）或乙酸乙酯（用于测定萘普生和去甲基萘普生）萃取，蒸发有机溶剂，将残留物溶解于乙腈中，然后注入与紫外吸收检测器相连的反相柱。3. 在0.5毫升样品中，浓度高于每毫升2微克时，药物和代谢物能够很容易地被检测到。发现水杨酸及其代谢物以及许多其他药物不会干扰该测定。