用高放射性的125-I缀合物清除半抗原结合细胞。
Deletion of hapten-binding cells by a highly radioactive 125-I conjugate.
作者信息
Golan D T, Borel Y
出版信息
J Exp Med. 1972 Aug 1;136(2):305-17. doi: 10.1084/jem.136.2.305.
Abstract
Exposure of normal mouse spleen cells in vitro to highly (125)I-labeled dinitrophenyl (DNP)-protein carrier conjugates specifically inactivated cells able to mount an immune response to that hapten after in vivo challenge. The deletion was hapten specific and independent of the radioactive carrier to which the hapten was bound. DNP-binding cells were inactivated by radioactivity that was not part of the hapten, but was solely confined to the carrier moiety. The deletion of the anti-DNP response lasted 2-3 wk and could be specifically inhibited.
摘要
体外将正常小鼠脾细胞暴露于高度（125）I标记的二硝基苯基（DNP）-蛋白质载体缀合物中，可特异性灭活那些在体内受到攻击后能够对该半抗原产生免疫反应的细胞。这种缺失是半抗原特异性的，且与半抗原所结合的放射性载体无关。DNP结合细胞被并非半抗原一部分、而是仅局限于载体部分的放射性所灭活。抗DNP反应的缺失持续2至3周，并且可以被特异性抑制。
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