Golan D T, Borel Y
J Exp Med. 1971 Oct 1;134(4):1046-61. doi: 10.1084/jem.134.4.1046.
Treatment of adult mice with dinitrophenyl (DNP) bound to isogeneic serum resulted in a specific inability to respond to DNP after challenge with DNP-keyhole limpet hemocyanin (KLH) in complete Freund's adjuvant. The unresponsiveness to the hapten had all the characteristics of immunologic tolerance: it had a definite induction time; it was transient but could be maintained by additional injections of the tolerogen; it was antigen specific and dose dependent. In addition, the induction of tolerance to DNP is dependent on the nature of the carrier. Two main conclusions can be drawn from these data: DNP conjugates of three homopolymers of lysine were found to be nonimmunogenic in mice, yet tolerogenic. Thus, antigenicity is not necessary to induce tolerance. Among the various carriers tested, isogeneic 7S immunoglobulin (IgG) was found to be the most effective to induce and maintain tolerance to the hapten. This suggests that IgG may have a function other than its usual role as an immunoglobulin.
用与同基因血清结合的二硝基苯基（DNP）处理成年小鼠，在用完全弗氏佐剂中的DNP-钥孔戚血蓝蛋白（KLH）攻击后，小鼠出现对DNP特异性无反应。对半抗原的无反应性具有免疫耐受的所有特征：有明确的诱导期；是短暂的，但可通过额外注射耐受原维持；具有抗原特异性且剂量依赖性。此外，对DNP耐受的诱导取决于载体的性质。从这些数据可得出两个主要结论：发现赖氨酸三种均聚物的DNP缀合物在小鼠中无免疫原性，但具有耐受性。因此，诱导耐受不需要抗原性。在测试的各种载体中，发现同基因7S免疫球蛋白（IgG）是诱导和维持对半抗原耐受最有效的。这表明IgG可能具有不同于其作为免疫球蛋白通常作用的功能。
