λ噬菌体形成噬菌斑、转导半乳糖菌株的分离
Isolation of plaque-forming, galactose-transducing strains of phage lambda.
作者信息
Feiss M, Adyha S, Court D L
出版信息
Genetics. 1972 Jun;71(2):189-206. doi: 10.1093/genetics/71.2.189.
Abstract
Plaque-forming, galactose-transducing lambda strains have been isolated from lysogens in which bacterial genes have been removed from between the galactose operon and the prophage by deletion mutation.-A second class has been isolated starting with a lysogenic strain which carries a deletion of the genes to the right of the galactose operon and part of the prophage. This strain was lysogenized with a second lambda phage to yield a lysogen from which galactose-transducing, plaque-forming phages were obtained. These plaque-forming phages were found to be genetically unstable, due to a duplication of part of the lambda chromosome. The genetic instability of these partial diploid strains is due to homologous genetic recombindation between the two identical copies of the phage DNA comprising the duplication. The galactose operon and the duplication of phage DNA carried by these strains is located between the phage lambda P and Q genes.
摘要
已从溶源菌中分离出形成噬菌斑、转导半乳糖的λ菌株，在这些溶源菌中，通过缺失突变已从半乳糖操纵子和原噬菌体之间去除了细菌基因。第二类菌株是从一个溶源菌株开始分离得到的，该溶源菌株携带半乳糖操纵子右侧基因和部分原噬菌体的缺失。用第二种λ噬菌体使该菌株溶源化，产生一个溶源菌，从中获得转导半乳糖、形成噬菌斑的噬菌体。由于λ染色体部分重复，发现这些形成噬菌斑的噬菌体在遗传上不稳定。这些部分二倍体菌株的遗传不稳定性是由于构成重复的噬菌体DNA的两个相同拷贝之间的同源基因重组。这些菌株携带的半乳糖操纵子和噬菌体DNA重复位于噬菌体λ P基因和Q基因之间。
相似文献
1
Isolation of plaque-forming, galactose-transducing strains of phage lambda.λ噬菌体形成噬菌斑、转导半乳糖菌株的分离
Genetics. 1972 Jun;71(2):189-206. doi: 10.1093/genetics/71.2.189.
2
Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages.大肠杆菌染色体中乳糖操纵子区域转位至半乳糖操纵子区域：λ-乳糖转导噬菌体的分离
J Bacteriol. 1971 Oct;108(1):5-9. doi: 10.1128/jb.108.1.5-9.1971.
3
Insertion of bacteriophage lambda into the deo operon of Escherichia coli K-12 and isolation of plaque-forming lambdadeo+ transducing bacteriophages.将噬菌体λ插入大肠杆菌K-12的deo操纵子并分离形成噬菌斑的λdeo⁺转导噬菌体。
J Bacteriol. 1978 Nov;136(2):668-81. doi: 10.1128/jb.136.2.668-681.1978.
4
Directed integration of an F' plasmid by integrative suppression: isolation of plaque forming lambda transducing phage for the dnaC gene.
Mol Gen Genet. 1977 Oct 20;155(2):153-62. doi: 10.1007/BF00393154.
5
Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r.携带大肠杆菌B/r L-阿拉伯糖操纵子基因的特异性转导噬菌体λ的分离
J Bacteriol. 1975 Sep;123(3):1043-54. doi: 10.1128/jb.123.3.1043-1054.1975.
6
Specialized transduction of galactose by lambda phage from a deletion lysogen.来自缺失溶原菌的λ噬菌体对半乳糖的特异性转导
Virology. 1970 Jul;41(3):474-87. doi: 10.1016/0042-6822(70)90169-8.
7
Isolation and characterization of lambda pleu bacteriophages.λ 类噬菌体的分离与特性分析
J Bacteriol. 1977 Feb;129(2):1078-90. doi: 10.1128/jb.129.2.1078-1090.1977.
8
Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12.用于大肠杆菌K-12鞭毛基因（fla）的特异性λ转导噬菌体的分离
J Virol. 1977 Jun;22(3):654-61. doi: 10.1128/JVI.22.3.654-661.1977.
9
Isolation and characterization of plaque-forming lambdadnaZ+ transducing bacteriophages.噬菌斑形成的λdnaZ⁺转导噬菌体的分离与鉴定
J Bacteriol. 1977 Apr;130(1):354-65. doi: 10.1128/jb.130.1.354-365.1977.
10
Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli.用于大肠杆菌su1 +（supD减）琥珀抑制基因的λ转导噬菌体的分离与鉴定。
J Bacteriol. 1975 Apr;122(1):120-8. doi: 10.1128/jb.122.1.120-128.1975.
引用本文的文献
1
The nature of the gal3 mutation of Escherichia coli.大肠杆菌gal3突变的性质。
Mol Gen Genet. 1975;136(3):233-42. doi: 10.1007/BF00334018.
2
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.鉴定大肠杆菌K-12的ybhE基因即为pgl，其编码6-磷酸葡萄糖酸内酯酶。
J Bacteriol. 2004 Dec;186(24):8248-53. doi: 10.1128/JB.186.24.8248-8253.2004.
3
Regulation of expression of the 2-deoxy-D-ribose utilization regulon, deoQKPX, from Salmonella enterica serovar typhimurium.鼠伤寒沙门氏菌2-脱氧-D-核糖利用操纵子deoQKPX表达的调控
J Bacteriol. 2003 Oct;185(20):6042-50. doi: 10.1128/JB.185.20.6042-6050.2003.
4
The nac (nitrogen assimilation control) gene from Escherichia coli.来自大肠杆菌的nac（氮同化控制）基因。
J Bacteriol. 1998 Mar;180(5):1166-73. doi: 10.1128/JB.180.5.1166-1173.1998.
5
Chi activity during transduction-associated recombination.转导相关重组过程中的Chi活性。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7033-7. doi: 10.1073/pnas.78.11.7033.
6
Role of sulA and sulB in filamentation by lon mutants of Escherichia coli K-12.sulA和sulB在大肠杆菌K-12 lon突变体丝状化中的作用。
J Bacteriol. 1981 Oct;148(1):265-73. doi: 10.1128/jb.148.1.265-273.1981.
7
Suppression of polarity in the gal operon by ultraviolet irradiation.紫外线照射对半乳糖操纵子极性的抑制作用。
J Bacteriol. 1980 Dec;144(3):1205-7. doi: 10.1128/jb.144.3.1205-1207.1980.
8
DNA-stimulated ATPase activity on the lon (CapR) protein.lon（CapR）蛋白上的DNA刺激的ATP酶活性。
J Bacteriol. 1984 Apr;158(1):195-201. doi: 10.1128/jb.158.1.195-201.1984.
9
Transcription initiation sites within an IS2 insertion in a Gal-constitutive mutant of Escherichia coli.大肠杆菌半乳糖组成型突变体中IS2插入片段内的转录起始位点。
Nucleic Acids Res. 1982 Aug 25;10(16):5015-31. doi: 10.1093/nar/10.16.5015.
10
Posttranscriptional control of bacteriophage lambda gene expression from a site distal to the gene.噬菌体λ基因表达在远离该基因的位点的转录后调控。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):238-42. doi: 10.1073/pnas.79.2.238.
本文引用的文献
1
Transduction and segregation in Escherichia coli K12.大肠杆菌K12中的转导与分离
Virology. 1957 Oct;4(2):366-84. doi: 10.1016/0042-6822(57)90070-3.
2
Partial diploidy in phage T41.噬菌体T41中的部分二倍体。
Genetics. 1965 Sep;52(3):683-93. doi: 10.1093/genetics/52.3.683.
3
Integration-negative mutants of bacteriophage lambda.噬菌体λ的整合阴性突变体
J Mol Biol. 1968 Feb 14;31(3):487-505. doi: 10.1016/0022-2836(68)90423-3.
4
Isolation and characterization of new sus (amber) mutants of bacteriophage lambda.噬菌体λ新sus（琥珀）突变体的分离与鉴定
Mutat Res. 1967 Nov-Dec;4(6):735-41. doi: 10.1016/0027-5107(67)90082-6.
5
Galactose 1-phosphate uridylyltransferase of Escherichia coli. II. Further purification and characterization.
J Biol Chem. 1967 May 25;242(10):2362-8.
6
Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.大肠杆菌中芳香族生物合成第一步反应所涉及的同工酶的遗传与生化分析。
J Bacteriol. 1967 Jan;93(1):237-44. doi: 10.1128/jb.93.1.237-244.1967.
7
A deletion analysis of prophage lambda and adjacent genetic regions.噬菌体λ及相邻基因区域的缺失分析
Proc Natl Acad Sci U S A. 1968 Nov;61(3):956-62. doi: 10.1073/pnas.61.3.956.
8
Integrative and excisive recombination by bacteriophage lambda: evidence for an excision-specific recombination protein.噬菌体λ的整合与切除重组：存在切除特异性重组蛋白的证据
J Mol Biol. 1970 Feb 14;47(3):575-83. doi: 10.1016/0022-2836(70)90324-4.
9
Isolation of an E. coli strain with a mutation affecting DNA polymerase.分离出一株具有影响DNA聚合酶突变的大肠杆菌菌株。
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
10
Suppressor gene Su3+ of E. coli, a structural gene for tyrosine TRNA.大肠杆菌的抑制基因Su3 +，一种酪氨酸tRNA的结构基因。
Proc Natl Acad Sci U S A. 1968 Mar;59(3):792-9. doi: 10.1073/pnas.59.3.792.
Zotero 插件