酵母中的转录：多种FNA聚合酶的分离与特性
Transcription in yeast: separation and properties of multiple FNA polymerases.
作者信息
Adman R, Schultz L D, Hall B D
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702-6. doi: 10.1073/pnas.69.7.1702.
Abstract
Four peaks of DNA-directed RNA polymerase activity are resolved by salt gradient elution of a sonicated yeast cell extract on DEAE-Sephadex. The enzymes, which are named IA, IB, II, and III in order of elution, all appear to come from cell nuclei. Only enzyme II is sensitive to alpha-amanitin. All enzymes are more active with Mn(++) than with Mg(++) as divalent ion. Enzymes IB and II have salt optima in the range 0.05-0.10 M (NH(4))(2)SO(4), whereas enzyme III is maximally active at 0.20-0.25 M (NH(4))(2)SO(4). With optimal salt concentration and saturating DNA, the template preference ratio, activity on native calfthymus DNA divided by activity on denatured calf-thymus DNA, is 2.2 for IB, 0.4 for II, and 3.5 for III. None of the yeast polymerases was inhibited by rifamycin SV. Rifamycin AF/013 effectively inhibited polymerases IB, II, and III.
摘要
通过在DEAE-葡聚糖凝胶上对超声破碎的酵母细胞提取物进行盐梯度洗脱，可分离出DNA指导的RNA聚合酶活性的四个峰。这些酶按照洗脱顺序分别命名为IA、IB、II和III，似乎都来自细胞核。只有酶II对α-鹅膏蕈碱敏感。作为二价离子，所有酶在以Mn(++)作为离子时比以Mg(++)时更具活性。酶IB和II的盐最适浓度范围为0.05 - 0.10M硫酸铵，而酶III在0.20 - 0.25M硫酸铵时活性最高。在最佳盐浓度和饱和DNA条件下，模板偏好率（天然小牛胸腺DNA上的活性除以变性小牛胸腺DNA上的活性），IB为2.2，II为0.4，III为3.5。利福霉素SV对酵母聚合酶均无抑制作用。利福霉素AF/013能有效抑制聚合酶IB、II和III。
相似文献
1
Transcription in yeast: separation and properties of multiple FNA polymerases.酵母中的转录：多种FNA聚合酶的分离与特性
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702-6. doi: 10.1073/pnas.69.7.1702.
2
RNA polymerases of maize: nuclear RNA polymerases.玉米的RNA聚合酶：核RNA聚合酶
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2647-51. doi: 10.1073/pnas.68.11.2647.
3
Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.酵母中的转录：α-鹅膏蕈碱敏感性以及区分RNA聚合酶I和III的其他特性。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1029-33. doi: 10.1073/pnas.73.4.1029.
4
Isolation of a distinct rifampicin-resistant RNA polymerase from mitochondria of yeast, neurospora and liver.从酵母、脉孢菌和肝脏的线粒体中分离出一种独特的耐利福平RNA聚合酶。
Biochem Biophys Res Commun. 1972 Sep 5;48(5):1287-94. doi: 10.1016/0006-291x(72)90851-0.
5
Solubilization and partial characterization of soybean chromatin-bound RNA polymerase.大豆染色质结合RNA聚合酶的溶解及部分特性分析
Biochem Biophys Res Commun. 1972 Jul 25;48(2):299-306. doi: 10.1016/s0006-291x(72)80050-0.
6
DNA-dependent RNA polymerases of Ehrlich carcinoma, other murine ascites tumors, and murine normal tissues.艾氏癌、其他小鼠腹水瘤及小鼠正常组织的依赖DNA的RNA聚合酶
J Natl Cancer Inst. 1975 Aug;55(2):397-412. doi: 10.1093/jnci/55.2.397.
7
In vivo and in vitro effects of rifampicin and streptolydigin on transcription of Kluyveromyces lactis in the presence of nystatin.在制霉菌素存在的情况下，利福平与链霉溶菌素对乳酸克鲁维酵母转录的体内和体外效应
Nucleic Acids Res. 1975 Feb;2(2):239-55. doi: 10.1093/nar/2.2.239.
8
Inhibition of yeast ribonucleic acid polymerases by thiolutin.硫藤黄素对酵母核糖核酸聚合酶的抑制作用。
J Bacteriol. 1973 Oct;116(1):245-56. doi: 10.1128/jb.116.1.245-256.1973.
9
DNA-dependent RNA polymerase from Tetrahymena pyriformis.来自梨形四膜虫的依赖DNA的RNA聚合酶。
Can J Biochem. 1972 Feb;50(2):154-7. doi: 10.1139/o72-020.
10
Purification and properties of two RNA polymerases from Physarum polycephalum.多头绒泡菌两种RNA聚合酶的纯化及性质
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1174-7. doi: 10.1073/pnas.71.4.1174.
引用本文的文献
1
Dynamic structures of intrinsically disordered proteins related to the general transcription factor TFIIH, nucleosomes, and histone chaperones.与通用转录因子TFIIH、核小体和组蛋白伴侣相关的内在无序蛋白质的动态结构。
Biophys Rev. 2022 Nov 18;14(6):1449-1472. doi: 10.1007/s12551-022-01014-9. eCollection 2022 Dec.
2
A mutation in POLR3E impairs antiviral immune response and RNA polymerase III.POLR3E 突变会损害抗病毒免疫反应和 RNA 聚合酶 III。
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22113-22121. doi: 10.1073/pnas.2009947117. Epub 2020 Aug 25.
3
A multistep process gave rise to RNA polymerase IV of land plants.一个多步骤过程产生了陆地植物的RNA聚合酶IV。
J Mol Evol. 2007 Jan;64(1):101-12. doi: 10.1007/s00239-006-0093-z. Epub 2006 Dec 9.
4
Purification and Characterization of DNA-dependent RNA Polymerases from Cauliflower Nuclei.来自花椰菜细胞核的依赖DNA的RNA聚合酶的纯化与特性分析
Plant Physiol. 1976 Oct;58(4):453-8. doi: 10.1104/pp.58.4.453.
5
Rifampin: inhibition of ribonucleic acid synthesis after potentiation by amphotericin B in Saccharomyces cerevisiae.利福平：在酿酒酵母中经两性霉素B增强作用后对核糖核酸合成的抑制作用。
Antimicrob Agents Chemother. 1974 Apr;5(4):371-6. doi: 10.1128/AAC.5.4.371.
6
RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.RNA聚合酶II在恶性疟原虫中合成反义RNA。
RNA. 2005 Apr;11(4):365-70. doi: 10.1261/rna.7940705. Epub 2005 Feb 9.
7
Similar upstream regulatory elements of genes that encode the two largest subunits of RNA polymerase II in Saccharomyces cerevisiae.酿酒酵母中编码RNA聚合酶II两个最大亚基的基因的类似上游调控元件。
Nucleic Acids Res. 1996 Nov 15;24(22):4543-51. doi: 10.1093/nar/24.22.4543.
8
An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.来自酿酒酵母的体外RNA聚合酶III系统：缺失和点突变对SUP4基因转录的影响。
Nucleic Acids Res. 1982 Dec 20;10(24):8127-43. doi: 10.1093/nar/10.24.8127.
9
Effects of rifamycin derivatives, alone and in combination with amphotericin B, against Histoplasma capsulatum.利福霉素衍生物单独及与两性霉素B联合使用对荚膜组织胞浆菌的作用。
Antimicrob Agents Chemother. 1974 Jan;5(1):16-8. doi: 10.1128/AAC.5.1.16.
10
Ribonucleic acid polymerases of the yeast phase of Histoplasma capsulatum.荚膜组织胞浆菌酵母相的核糖核酸聚合酶
J Bacteriol. 1974 May;118(2):480-5. doi: 10.1128/jb.118.2.480-485.1974.
本文引用的文献
1
Role of DNA-RNA hybrids in eukaryots 1. Purification of yeast RNA polymerase B.DNA - RNA 杂交体在真核生物中的作用 1. 酵母 RNA 聚合酶 B 的纯化。
FEBS Lett. 1972 Mar;21(1):1-6. doi: 10.1016/0014-5793(72)80148-0.
2
Structural evidence for two alpha-amanitin sensitive RNA polymerases in calf thymus.小牛胸腺中两种对α-鹅膏蕈碱敏感的RNA聚合酶的结构证据。
FEBS Lett. 1971 Jun 24;15(3):169-174. doi: 10.1016/0014-5793(71)80305-8.
3
Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.从分离的亚基重构细菌DNA依赖性RNA聚合酶作为阐明亚基在转录中作用的工具。
FEBS Lett. 1970 Dec;11(3):165-168. doi: 10.1016/0014-5793(70)80519-1.
4
Biochemical correlates of respiratory deficiency. VII. Glucose repression.呼吸缺陷的生化关联。VII. 葡萄糖阻遏。
Arch Biochem Biophys. 1966 Sep 26;116(1):224-51. doi: 10.1016/0003-9861(66)90029-4.
5
The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants.酿酒酵母中孢子形成的遗传控制。I. 温度敏感型孢子形成缺陷突变体的分离。
Genetics. 1969 Jan;61(1):79-89. doi: 10.1093/genetics/61.1.79.
6
Genetic mapping of nonsense suppressors in yeast.酵母中无义抑制基因的遗传图谱
Genetics. 1968 Dec;60(4):735-42. doi: 10.1093/genetics/60.4.735.
7
Function of T4 gene 55. II. RNA synthesis by temperature-sensitive gene 55 mutants.T4基因55的功能。二、温度敏感型基因55突变体的RNA合成。
J Mol Biol. 1970 Apr 28;49(2):489-507. doi: 10.1016/0022-2836(70)90259-7.
8
Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.真核生物中DNA依赖性RNA聚合酶的多种形式。
Nature. 1969 Oct 18;224(5216):234-7. doi: 10.1038/224234a0.
9
Macromolecule synthesis in temperature-sensitive mutants of yeast.酵母温度敏感突变体中的大分子合成
J Bacteriol. 1967 May;93(5):1662-70. doi: 10.1128/jb.93.5.1662-1670.1967.
10
Molecular structures of DNA-dependent RNA polymerases (II) from calf thymus and rat liver.来自小牛胸腺和大鼠肝脏的DNA依赖性RNA聚合酶（II）的分子结构。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2994-9. doi: 10.1073/pnas.68.12.2994.
Zotero 插件