Tseng B Y, Marvin D A
J Virol. 1972 Sep;10(3):392-8. doi: 10.1128/JVI.10.3.392-398.1972.
Synthesis of fd deoxyribonucleic acid (DNA) was stopped by transferring infected bacteria from 32 C into chloramphenicol or serine hydroxamate at 42 C, but not by addition of these antibiotics at 32 C, and not by a temperature change in the absence of antibiotics. The inhibition of fd DNA synthesis by serine hydroxamate at 42 C was reversed by excess serine. The ability to synthesize fd DNA at 42 C in chloramphenicol was rescued by delaying the addition of chloramphenicol for a few minutes after the transfer from 32 to 42 C. The colony-forming ability of abortively infected bacteria was also rescued from "killing" by delaying the addition of chloramphenicol after a transfer from 32 to 42 C.
通过将受感染细菌从32℃转移至42℃的氯霉素或丝氨酸异羟肟酸中，fd脱氧核糖核酸（DNA）的合成被阻断，但在32℃添加这些抗生素则不会阻断，且在无抗生素情况下改变温度也不会阻断。42℃时丝氨酸异羟肟酸对fd DNA合成的抑制作用可被过量丝氨酸逆转。在从32℃转移至42℃后延迟几分钟添加氯霉素，可挽救在42℃氯霉素中合成fd DNA的能力。通过在从32℃转移至42℃后延迟添加氯霉素，流产感染细菌的集落形成能力也可从“杀伤”中挽救回来。
