Szargel R, Shalitin C
J Virol. 1972 Aug;10(2):310-3. doi: 10.1128/JVI.10.2.310-313.1972.
Sedimentation of the replicative deoxyribonucleic acid through alkaline sucrose gradients showed that rII single chains reached the half-mature size at a time when wild-type molecules formed long chains (dimers and trimers of genome size). Long rII single chains could be observed on substitution of tris(hydroxymethyl)aminomethane buffer for Na(+)K(+) phosphate in the growth medium.
通过碱性蔗糖梯度对复制性脱氧核糖核酸进行沉降分析表明,当野生型分子形成长链(基因组大小的二聚体和三聚体)时,rII单链达到了半成熟大小。在生长培养基中用三(羟甲基)氨基甲烷缓冲液替代磷酸钠钾时,可以观察到rII长单链。