基因30（脱氧核糖核酸连接酶）和rII基因缺陷的噬菌体T4突变体的特性
Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.
作者信息
Karam J D, Barker B
出版信息
J Virol. 1971 Feb;7(2):260-6. doi: 10.1128/JVI.7.2.260-266.1971.
Abstract
In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled with temperature shifts and with inhibition of DNA synthesis, it was observed that DNA synthesized by gene 30-defective phage is more susceptible to breakdown in vivo when the phage is carrying a wild-type rII gene. Breakdown was delayed or inhibited by continued DNA synthesis. Mutations of the rII gene decreased but did not completely abolish the breakdown. T4 ligase-rII double mutants had normal sensitivity to ultraviolet irradiation.
摘要
在感染了基因30（脱氧核糖核酸（DNA）连接酶）和rII基因（产物未知）有缺陷的噬菌体T4的大肠杆菌K-12菌株中，产生了接近正常水平的DNA和有活力的噬菌体。这种T4连接酶-rII双突变体在表现出rII突变的“快速裂解”表型的大肠杆菌B菌株中的生长效率较低。在结合温度变化和DNA合成抑制的脉冲追踪实验中，观察到当携带野生型rII基因时，由基因30缺陷型噬菌体合成的DNA在体内更易被分解。持续的DNA合成可延迟或抑制分解。rII基因的突变减少了但并未完全消除分解。T4连接酶-rII双突变体对紫外线照射具有正常的敏感性。
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