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分枝杆菌凝集抑制试验。

Mycobacterial agglutination-inhibition test.

作者信息

Richards W D, Eacret W G

出版信息

Appl Microbiol. 1972 Sep;24(3):318-22. doi: 10.1128/am.24.3.318-322.1972.

DOI:10.1128/am.24.3.318-322.1972
PMID:4562472
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376517/
Abstract

The mycobacterial agglutination-inhibition test (MAIT) is described as a modification of the mycobacterial agglutination of Schaefer (MATS). It is an organized assemblage of absorption and agglutination tests which is especially useful in the identification of Mycobacterium avium isolates normally excluded from serological identification because of unstable cell suspension characteristics. The identity of 100 unstable M. avium and 3 unstable Mycobacterium intracellulare isolates was determined by the MAIT method and by animal pathogenicity tests. The comparability of the MAIT and MATS methods was demonstrated by testing 10 stable M. avium and 10 stable M. intracellulare isolates by both methods and obtaining the same serological identification for each culture. The reproducibility of the MAIT was confirmed when the same result was obtained in three consecutive tests on each of the 123 cultures used in this evaluation.

摘要

分枝杆菌凝集抑制试验(MAIT)被描述为对舍费尔分枝杆菌凝集试验(MATS)的一种改良。它是吸收试验和凝集试验的有序组合,对于鉴定通常因细胞悬液特性不稳定而被血清学鉴定排除在外的鸟分枝杆菌分离株特别有用。通过MAIT方法和动物致病性试验确定了100株不稳定的鸟分枝杆菌和3株不稳定的胞内分枝杆菌分离株的身份。通过用两种方法对10株稳定的鸟分枝杆菌和10株稳定的胞内分枝杆菌分离株进行检测,并对每种培养物获得相同的血清学鉴定结果,证明了MAIT和MATS方法的可比性。当对本评估中使用的123种培养物中的每一种进行连续三次测试都得到相同结果时,证实了MAIT的可重复性。

相似文献

1
Mycobacterial agglutination-inhibition test.分枝杆菌凝集抑制试验。
Appl Microbiol. 1972 Sep;24(3):318-22. doi: 10.1128/am.24.3.318-322.1972.
2
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Production of multivalent fluorescent antisera for identification of organisms in the Mycobacterium avium-Mycobacterium intracellulare complex.用于鉴定鸟分枝杆菌-胞内分枝杆菌复合群中细菌的多价荧光抗血清的制备。
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本文引用的文献

1
Type-specificity of atypical mycobacteria in agglutination and antibody absorption tests.
Am Rev Respir Dis. 1967 Dec;96(6):1165-8. doi: 10.1164/arrd.1967.96.6.1165.
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Serologic identification and classification of the atypical mycobacteria by their agglutination.通过凝集反应对非典型分枝杆菌进行血清学鉴定和分类。
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Incidence of the serotypes of Mycobacerium avium and atypical mycobacteria in human and animal diseases.鸟分枝杆菌血清型及非典型分枝杆菌在人类和动物疾病中的发病率。
Am Rev Respir Dis. 1968 Jan;97(1):18-23. doi: 10.1164/arrd.1968.97.1.18.
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Comparison of the seroagglutination test with the pathogenicity test in the chicken for the identification of Mycobacterium avium and Mycobacterium intracellulare.
Am Rev Respir Dis. 1971 Feb;103(2):173-8. doi: 10.1164/arrd.1971.103.2.173.
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Identification and incidence of a third type of Mycobacterium avium.第三种鸟分枝杆菌的鉴定与发病率
Tubercle. 1969 Dec;50(4):394-5.
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Mycobacterium avium. A bacteriological and epidemiological study of M. avium isolated from animals and man in Denmark. 2. Strains isolated from man.
Acta Pathol Microbiol Scand. 1968;72(2):295-312.