Gales P W, Martins R R, Walker W E
Appl Microbiol. 1974 Apr;27(4):753-5. doi: 10.1128/am.27.4.753-755.1974.
Antisera to ten strains of mycobacteria in the Mycobacterium avium-Mycobacterium intracellulare group were obtained by injecting rabbits with ultraviolet light-killed cells. The antisera were conjugated with fluorescein isothiocyanate and used in the direct fluorescent antibody test. Individual antisera reacted specifically with the mycobacterial serotype used to produce them. The antisera were then combined in two multivalent pools. Each multivalent pool reacted specifically with its corresponding antigens. The multivalent antisera were thus found to provide a rapid identification method for the mycobacteria studied.
通过向兔子注射经紫外线灭活的细胞，获得了针对鸟分枝杆菌-胞内分枝杆菌组中十种分枝杆菌菌株的抗血清。将这些抗血清与异硫氰酸荧光素结合，并用于直接荧光抗体试验。单个抗血清与用于产生它们的分枝杆菌血清型发生特异性反应。然后将这些抗血清合并成两个多价组合。每个多价组合都与其相应的抗原发生特异性反应。因此，发现多价抗血清为所研究的分枝杆菌提供了一种快速鉴定方法。
