Brenner D J, Fanning G R, Steigerwalt A G, Orskov I, Orskov F
Infect Immun. 1972 Sep;6(3):308-15. doi: 10.1128/iai.6.3.308-315.1972.
Escherichia coli strains that cause dysentery-like disease, parenteral infection, and infantile diarrhea form specific groups based on mobility of O and K antigens in immunoelectrophoresis. Members from each of these groups were assayed for gross nucleotide sequence relatedness. The method used was interspecific deoxyribonucleic acid (DNA) reassociation reactions carried out free in solution. Reassociated DNA was separated from unreacted DNA by passage through hydroxyapatite. DNA relatedness between these groups was approximately 80%. The groups containing those strains causing parenteral infection and those responsible for dysentery-like disease showed preferentially high intragroup DNA relatedness. The group containing strains responsible for infantile diarrhea did not show preferentially high intragroup DNA relatedness with the reference strain employed. These strains, however, did exhibit preferentially high DNA relatedness to a second reference strain.
引起痢疾样疾病、肠道外感染及婴儿腹泻的大肠杆菌菌株，根据O和K抗原在免疫电泳中的迁移率形成特定的菌群。对这些菌群中的每个成员进行了总核苷酸序列相关性分析。所采用的方法是在溶液中进行种间脱氧核糖核酸（DNA）重缔合反应。通过羟基磷灰石柱将重缔合的DNA与未反应的DNA分离。这些菌群之间的DNA相关性约为80%。包含引起肠道外感染的菌株和引起痢疾样疾病的菌株的菌群，在菌群内显示出优先的高DNA相关性。包含引起婴儿腹泻的菌株的菌群，与所采用的参考菌株相比，并未显示出优先的高菌群内DNA相关性。然而，这些菌株与第二个参考菌株显示出优先的高DNA相关性。
