布鲁氏菌脱氧核糖核酸的多核苷酸同源性
Polynucleotide homologies of Brucella deoxyribonucleic acids.
作者信息
Hoyer B H, McCullough N B
出版信息
J Bacteriol. 1968 Feb;95(2):444-8. doi: 10.1128/jb.95.2.444-448.1968.
Abstract
Deoxyribonucleic acids (DNA's) extracted from organisms presently placed in the genus Brucella (B. abortus, B. melitensis, B. neotomae, and B. suis) possessed very similar polynucleotide sequences. Unlabeled, single-stranded DNA fragments from B. abortus, B. melitensis, B. neotomae, and B. suis were equally effective in competing with the interaction of corresponding radiolabeled, single-stranded DNA fragments with their homologous DNA-agars. Unlabeled fragments of B. ovis, however, did not compete as effectively as the homologous, unlabeled DNA's, and this organism, therefore, had a detectably different polynucleotide composition. The mole percentages of guanine plus cytosine in Brucella DNA's (56 to 58%) were also similar. DNA's from Francisella tularensis, Escherichia coli, and the slow loris did not compete.
摘要
从目前归属于布鲁氏菌属的生物体（流产布鲁氏菌、羊布鲁氏菌、新墨西哥布鲁氏菌和猪布鲁氏菌）中提取的脱氧核糖核酸（DNA）具有非常相似的多核苷酸序列。来自流产布鲁氏菌、羊布鲁氏菌、新墨西哥布鲁氏菌和猪布鲁氏菌的未标记单链DNA片段在与相应放射性标记单链DNA片段与其同源DNA琼脂的相互作用竞争中同样有效。然而，绵羊布鲁氏菌的未标记片段在竞争中不如同源未标记DNA有效，因此，这种生物体具有可检测到的不同多核苷酸组成。布鲁氏菌DNA中鸟嘌呤加胞嘧啶的摩尔百分比（56%至58%）也相似。来自土拉热弗朗西斯菌、大肠杆菌和懒猴的DNA不参与竞争。
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