奈瑟菌属各物种间的脱氧核糖核酸同源性。
Deoxyribonucleic acid homologies among species of the genus Neisseria.
作者信息
Kingsbury D T
出版信息
J Bacteriol. 1967 Oct;94(4):870-4. doi: 10.1128/jb.94.4.870-874.1967.
Abstract
Eleven aerobic species of Neisseria, a Mima sp., and a Herellea sp. were tested for deoxyribonucleic acid (DNA) homology in direct hybridization experiments. DNA labeled with either (14)C or (32)P was prepared from five species of Neisseria. Unlabeled DNA from the various microorganisms was immobilized on membrane filters, which, after pretreatment, were incubated with labeled DNA (4,000 counts per min per filter) for 14 hr at 67 C. The measure of relatedness was expressed as the relative percentage of direct binding compared to that obtained with homologous DNA. All serological types of N. meningitidis, including the newly proposed types, were homologous to the standard strain of N. meningitidis with one possible exception, type Z. The genus Neisseria is heterogeneous in nature, forming at least three distinct groups: first, N. meningitidis and N. gonorrhoeae; second, N. perflava, N. subflava, N. sicca, N. flavescens, and N. flava; third, N. catarrhalis and N. caviae. Mima and Herellea species show no significant homology with the Neisseria.
摘要
在直接杂交实验中，对11种需氧奈瑟菌属细菌、1种米玛菌属细菌和1种赫雷勒菌属细菌进行了脱氧核糖核酸（DNA）同源性检测。从5种奈瑟菌属细菌中制备了用（14）C或（32）P标记的DNA。将来自各种微生物的未标记DNA固定在膜滤器上，经过预处理后，与标记的DNA（每个滤器每分钟4000计数）在67℃下孵育14小时。相关性的度量表示为与同源DNA相比直接结合的相对百分比。脑膜炎奈瑟菌的所有血清型，包括新提出的血清型，除了可能的Z型外，均与脑膜炎奈瑟菌标准菌株同源。奈瑟菌属本质上是异质的，形成至少三个不同的组：第一组，脑膜炎奈瑟菌和淋病奈瑟菌；第二组，浅黄奈瑟菌、微黄奈瑟菌、干燥奈瑟菌、变黄奈瑟菌和金黄奈瑟菌；第三组，卡他莫拉菌和豚鼠奈瑟菌。米玛菌属和赫雷勒菌属细菌与奈瑟菌属没有明显的同源性。
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