大肠杆菌严谨型菌株中的温度敏感松弛表型
Temperature-sensitive relaxed Phenotype in a stringent strain of Escherichia coli.
作者信息
Atherly A G
出版信息
J Bacteriol. 1973 Jan;113(1):178-82. doi: 10.1128/jb.113.1.178-182.1973.
Abstract
A temperature-sensitive elongation factor G (EF-G) mutation carried by a rel strain of Escherichia coli was transferred to a rel(+) strain. The recombinant (AA-10) was found to be temperature-sensitive "relaxed" for ribonucleic acid (RNA) regulation. However, when a temperature-sensitive EF-G is present in a strain temperature-sensitive for valyl-transfer RNA (tRNA) synthetase, this new strain (AA-16) is no longer temperature-sensitive "relaxed" for RNA regulation. In strain AA-10, all tRNAs remain fully charged, and in AA-16 tRNA(val) becomes completely discharged at the nonpermissive growth temperature. Also, synthesis of ppGpp is not observed in strain AA-10 at 42 C but is observed at 42 C in strain AA-16.
摘要
将一株携带温度敏感型延伸因子G（EF-G）突变的大肠杆菌rel菌株的突变基因转移到rel(+)菌株中。发现重组菌株（AA-10）在核糖核酸（RNA）调控方面对温度敏感且呈现“松弛”状态。然而，当在一株对缬氨酰转移RNA（tRNA）合成酶温度敏感的菌株中存在温度敏感型EF-G时，这个新菌株（AA-16）在RNA调控方面不再对温度敏感且呈现“松弛”状态。在AA-10菌株中，所有tRNA都保持完全充电状态，而在AA-16菌株中，在非允许生长温度下tRNA(val)会完全放电。此外，在42℃时，AA-10菌株中未观察到ppGpp的合成，但在42℃时AA-16菌株中可观察到ppGpp的合成。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1580-8. doi: 10.1073/pnas.47.10.1580.
2
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
3
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Biochem Biophys Res Commun. 1971 Feb 5;42(3):564-7. doi: 10.1016/0006-291x(71)90408-6.
4
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Biochem Biophys Res Commun. 1971 Feb 5;42(3):441-4. doi: 10.1016/0006-291x(71)90390-1.
5
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6
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7
8
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9
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10
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