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在无细胞、半合成软琼脂培养基中培养麻风分枝杆菌的尝试。

An attempt to culture Mycobacterium leprae in cell-free, semisynthetic, soft agar media.

作者信息

Murohashi T, Yoshida K

出版信息

Bull World Health Organ. 1972;47(2):195-210.

PMID:4566288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2480898/
Abstract

This paper describes an attempt to culture M. leprae from leprous nodules, using cell-free, semisynthetic, soft agar media, and the effects of varying the incubation temperature and the composition of the basic medium, as well as of pretreating the bacterial suspensions with alkali. The identification test carried out in leprosy patients by intradermal injection of bacillary suspensions prepared from the primary cultures and third subcultures suggested that the bacterial masses that had formed in the suspensions were composed of M. leprae.

摘要

本文描述了一项尝试，即使用无细胞、半合成软琼脂培养基从麻风结节中培养麻风分枝杆菌，以及改变培养温度、基础培养基成分的影响，以及用碱预处理细菌悬液的影响。通过对麻风患者进行皮内注射由原代培养物和第三代传代培养物制备的杆菌悬液所进行的鉴定试验表明，悬液中形成的细菌团块由麻风分枝杆菌组成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/a6600c8e5d1d/bullwho00182-0060-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/76db7c9173e0/bullwho00182-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/8999fa7d7105/bullwho00182-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/e4c81bb64171/bullwho00182-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/5b3f56b2cc25/bullwho00182-0059-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/9a2f755af349/bullwho00182-0059-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/7f4d7be6c01b/bullwho00182-0059-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/0338a1e44698/bullwho00182-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/afb2c29c265a/bullwho00182-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/d512f9bef741/bullwho00182-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/af2207ef0130/bullwho00182-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/5eafe8cf3ec2/bullwho00182-0061-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/1e58d831ee16/bullwho00182-0061-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/49f09757a1f6/bullwho00182-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/6d73cd688b66/bullwho00182-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/b7385058c1cc/bullwho00182-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/201f08c5503e/bullwho00182-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/1d1289a44e50/bullwho00182-0060-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/f21cdce2d60e/bullwho00182-0060-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/3feec2e3185a/bullwho00182-0060-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/a6600c8e5d1d/bullwho00182-0060-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/76db7c9173e0/bullwho00182-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/8999fa7d7105/bullwho00182-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/e4c81bb64171/bullwho00182-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/5b3f56b2cc25/bullwho00182-0059-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/9a2f755af349/bullwho00182-0059-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/7f4d7be6c01b/bullwho00182-0059-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/0338a1e44698/bullwho00182-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/afb2c29c265a/bullwho00182-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/d512f9bef741/bullwho00182-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/af2207ef0130/bullwho00182-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/5eafe8cf3ec2/bullwho00182-0061-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/1e58d831ee16/bullwho00182-0061-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/49f09757a1f6/bullwho00182-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/6d73cd688b66/bullwho00182-0062-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/b7385058c1cc/bullwho00182-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/201f08c5503e/bullwho00182-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/1d1289a44e50/bullwho00182-0060-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/f21cdce2d60e/bullwho00182-0060-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/3feec2e3185a/bullwho00182-0060-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff1/2480898/a6600c8e5d1d/bullwho00182-0060-f.jpg

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Bull World Health Organ. 1972;47(2):195-210.
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本文引用的文献

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Cytochemical studies on the differentiation of living and dead acid-fast bacilli.活的和死的抗酸杆菌分化的细胞化学研究。
Acta Tuberc Scand. 1957;34(3-4):208-25.
2
The relation of oxygen tension to virulence of tubercle bacilli and to acquired resistance in tuberculosis.氧张力与结核杆菌毒力及结核病获得性耐药性的关系。
J Infect Dis. 1957 Sep-Oct;101(2):193-202. doi: 10.1093/infdis/101.2.193.
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The metabolic properties of mycobacteria and the pathogenesis of mycobacterial disease.分枝杆菌的代谢特性与分枝杆菌病的发病机制。
Bibl Tuberc. 1956(10):1-16.
4
Virulence of the tubercle bacillus. II. Effect of oxygen tension upon growth of virulent and avirulent bacilli.结核杆菌的毒力。II. 氧张力对有毒和无毒杆菌生长的影响。
J Infect Dis. 1954 Jan-Feb;94(1):99-106. doi: 10.1093/infdis/94.1.99.
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Virulence of the tubercle bacillus. I. Effect of oxygen tension upon respiration of virulent and avirulent bacilli.结核杆菌的毒力。一、氧张力对有毒力和无毒力结核杆菌呼吸作用的影响。
J Infect Dis. 1954 Jan-Feb;94(1):90-8. doi: 10.1093/infdis/94.1.90.
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Limited multiplication of Mycobacterium lepraemurium in parabiotic culture, as influenced by osmolarity of an alkaline-galactomannan medium.碱性半乳甘露聚糖培养基的渗透压对副生物培养中鼠麻风分枝杆菌增殖的影响
J Bacteriol. 1966 May;91(5):1859-62. doi: 10.1128/jb.91.5.1859-1862.1966.
7
[Attempts of cultivation of Mycobacterium lepraemurium in the cell-free, semi-synthetic medium].[在无细胞半合成培养基中培养鼠麻风杆菌的尝试]
Nihon Saikingaku Zasshi. 1969 Apr;24(3):195-201.
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[Growth of M. paratuberculosis johnei in synthetic semiliquid-agar media without the addition of M. phlei].[副结核分枝杆菌约翰亚种在不添加草分枝杆菌的合成半液体琼脂培养基中的生长情况]
Igaku To Seibutsugaku. 1968 Jan 10;76(1):5-10.
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Biology of the mycobacterioses. Biological significance of acid-fastness of mycobacteria.
Ann N Y Acad Sci. 1968 Sep 5;154(1):58-67. doi: 10.1111/j.1749-6632.1968.tb16695.x.
10
Effect of ultraviolet irradiation on the acid-fastness of difficult to culture and unculturable mycobacteria.紫外线照射对难培养及不可培养分枝杆菌抗酸性的影响
Am Rev Respir Dis. 1968 Feb;97(2):306-10. doi: 10.1164/arrd.1968.97.2.306.