Murohashi T, Yoshida K
Bull World Health Organ. 1973 May;48(5):571-9.
A bacterial suspension prepared from a nodule from a patient with lepromatous leprosy was inoculated into the cell-free, semisynthetic, semiliquid agar medium M-Y 11d-modified from the authors' medium M-Y 1c by omitting glycerol and adding pyruvate-and incubated at 37 degrees C. The growth of the organism was stimulated so markedly that after about 50 weeks' incubation of the primary culture microcolonies could be seen floating in the agar layer as tiny white particles, while the medium fluid assumed a yellow tinge. These macroscopic colonies were sucked up by pipette and subjected to microscopic examination. A bacterial suspension prepared from the first subculture of this strain by centrifugation elicited the same skin reactions in leprosy patients as did the standard Mitsuda's antigen. The organism in the culture was therefore identified as M. leprae.
从一名瘤型麻风患者的结节制备的细菌悬液接种到无细胞、半合成、半液体琼脂培养基M-Y 11d中，该培养基是作者的培养基M-Y 1c通过省略甘油并添加丙酮酸而改良得到的，并在37℃下培养。该微生物的生长受到显著刺激，以至于在原代培养物培养约50周后，可以看到微菌落作为微小的白色颗粒漂浮在琼脂层中，而培养基液体呈现黄色。这些宏观菌落用移液管吸出并进行显微镜检查。通过离心从该菌株的首次传代培养物制备的细菌悬液在麻风患者中引起的皮肤反应与标准的 Mitsuda 抗原相同。因此，培养物中的微生物被鉴定为麻风分枝杆菌。
