鼠伤寒沙门氏菌转酮醇酶突变体中脂多糖和醛庚糖的生物合成
Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium.
作者信息
Eidels L, Osborn M J
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1673-7. doi: 10.1073/pnas.68.8.1673.
Abstract
Genetic and biochemical evidence that sedoheptulose-7-phosphate is an obligatory precursor of the L-glycero-D-mannoheptose residues of the lipopolysaccharide of Salmonella was obtained by isolation and characterization of transketolase-negative mutants of Salmonella typhimurium. These mutants, which are defective in synthesis of sedoheptulose-7-phosphate, were found to produce an incomplete heptose-deficient lipopolysaccharide, and were also sensitive to bile salts, a characteristic property of heptose-deficient mutants. Phenotypic repair of the defect in lipopolysaccharide synthesis was obtained by addition of exogenous sedoheptulose-7-phosphate to growing cultures of the mutant strains. Characterization of revertants isolated either as transketolase-positive or heptose-positive provided further evidence that the heptose deficiency resulted from mutation at the transketolase locus. On the basis of these findings a possible pathway for conversion of sedoheptulose-7-phosphate to L-glycero-D-mannoheptose is proposed.
摘要
通过对鼠伤寒沙门氏菌转酮醇酶阴性突变体的分离和鉴定，获得了遗传和生化证据，证明景天庚酮糖-7-磷酸是沙门氏菌脂多糖中L-甘油-D-甘露庚糖残基的必需前体。这些在景天庚酮糖-7-磷酸合成方面存在缺陷的突变体，被发现产生不完全的庚糖缺陷型脂多糖，并且对胆盐敏感，这是庚糖缺陷型突变体的一个特征性特性。通过向突变菌株的生长培养物中添加外源性景天庚酮糖-7-磷酸，获得了脂多糖合成缺陷的表型修复。对分离得到的转酮醇酶阳性或庚糖阳性回复突变体的鉴定提供了进一步的证据，表明庚糖缺陷是由转酮醇酶基因座的突变引起的。基于这些发现，提出了一条将景天庚酮糖-7-磷酸转化为L-甘油-D-甘露庚糖的可能途径。
相似文献
1
Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium.鼠伤寒沙门氏菌转酮醇酶突变体中脂多糖和醛庚糖的生物合成
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1673-7. doi: 10.1073/pnas.68.8.1673.
2
Leakage of periplasmic enzymes from lipopolysaccharide-defective mutants of Salmonella typhimurium.鼠伤寒沙门氏菌脂多糖缺陷型突变体中周质酶的泄漏。
Can J Microbiol. 1976 Oct;22(10):1549-60. doi: 10.1139/m76-227.
3
Rough mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide core.鼠伤寒沙门氏菌粗糙突变体，其脂多糖核心的庚糖区域存在缺陷。
Can J Microbiol. 1974 Aug;20(8):1127-34. doi: 10.1139/m74-175.
4
Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).鼠伤寒沙门氏菌突变体的分离，该突变体生长及合成3-脱氧-D-甘露辛酮糖酸（酮脱氧辛酮酸）依赖于D-阿拉伯糖-5-磷酸。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3756-60. doi: 10.1073/pnas.69.12.3756.
5
A new class of heptose-defective mutant of Salmonella typhimurium.
Eur J Biochem. 1973 Jan 15;32(2):268-75. doi: 10.1111/j.1432-1033.1973.tb02607.x.
6
LIPOPOLYSACCHARIDE OF THE GRAM-NEGATIVE CELL WALL.革兰氏阴性菌细胞壁的脂多糖
Science. 1964 Aug 21;145(3634):783-9. doi: 10.1126/science.145.3634.783.
7
Biosynthesis and structure of the core region of the lipopolysaccharide in Salmonella typhimurium.
Ann N Y Acad Sci. 1966 Jun 30;133(2):375-83. doi: 10.1111/j.1749-6632.1966.tb52377.x.
8
Biosynthesis of T1 antigen in Salmonella: origin of D-galactofuranose and D-ribofuranose residues.沙门氏菌中T1抗原的生物合成：D-半乳呋喃糖和D-核糖呋喃糖残基的起源
J Bacteriol. 1971 Mar;105(3):1063-72. doi: 10.1128/jb.105.3.1063-1072.1971.
9
Lipopolysaccharide core mutants of Salmonella typhimurium containing D-glycero-D-manno-heptose.含有D-甘油-D-甘露庚糖的鼠伤寒沙门氏菌脂多糖核心突变体。
J Bacteriol. 1983 Jun;154(3):1462-6. doi: 10.1128/jb.154.3.1462-1466.1983.
10
D-glycero-D-manno-heptose as a component of lipopolysaccharides from Gram-negative bacteria.D-甘油-D-甘露庚糖作为革兰氏阴性菌脂多糖的一个组成部分。
Can J Microbiol. 1967 Dec;13(12):1605-13. doi: 10.1139/m67-210.
引用本文的文献
1
Potentiating Activity of GmhA Inhibitors on Gram-Negative Bacteria.GmhA抑制剂对革兰氏阴性菌的增效活性。
J Med Chem. 2024 Apr 25;67(8):6610-6623. doi: 10.1021/acs.jmedchem.4c00037. Epub 2024 Apr 10.
2
Distinct Gut Microbiome Induced by Different Feeding Regimes in Weaned Piglets.不同断奶仔猪饲养方式引起的肠道微生物区系差异。
Genes (Basel). 2022 Dec 23;14(1):49. doi: 10.3390/genes14010049.
3
serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway.鼠伤寒血清型有三种转酮醇酶参与戊糖磷酸途径。
J Biol Chem. 2018 Jul 20;293(29):11271-11282. doi: 10.1074/jbc.RA118.003661. Epub 2018 May 30.
4
Functional and structural studies on the Neisseria gonorrhoeae GmhA, the first enzyme in the glycero-manno-heptose biosynthesis pathways, demonstrate a critical role in lipooligosaccharide synthesis and gonococcal viability.对淋病奈瑟菌GmhA（甘油甘露庚糖生物合成途径中的首个酶）的功能和结构研究表明，其在脂寡糖合成和淋球菌生存能力方面起着关键作用。
Microbiologyopen. 2017 Apr;6(2). doi: 10.1002/mbo3.432. Epub 2017 Jan 7.
5
Improvement of L-phenylalanine production from glycerol by recombinant Escherichia coli strains: the role of extra copies of glpK, glpX, and tktA genes.重组大肠杆菌菌株提高甘油生产L-苯丙氨酸的研究：glpK、glpX和tktA基因额外拷贝的作用
Microb Cell Fact. 2014 Jul 11;13(1):96. doi: 10.1186/s12934-014-0096-1.
6
Maize root lectins mediate the interaction with Herbaspirillum seropedicae via N-acetyl glucosamine residues of lipopolysaccharides.玉米根凝集素通过脂多糖的N-乙酰葡糖胺残基介导与血清巴西固氮螺菌的相互作用。
PLoS One. 2013 Oct 9;8(10):e77001. doi: 10.1371/journal.pone.0077001. eCollection 2013.
7
Identification of potential Leptospira phosphoheptose isomerase inhibitors through virtual high-throughput screening.通过虚拟高通量筛选鉴定潜在的钩端螺旋体磷酸庚糖异构酶抑制剂。
Genomics Proteomics Bioinformatics. 2010 Dec;8(4):246-55. doi: 10.1016/S1672-0229(10)60026-5.
8
The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism.Pfam家族PF06475首个代表成员的结构揭示了一种可能参与糖脂代谢的新折叠结构。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1211-7. doi: 10.1107/S1744309109022684. Epub 2009 Oct 27.
9
Gut microbial gene expression in mother-fed and formula-fed piglets.母源喂养和配方奶喂养仔猪的肠道微生物基因表达。
PLoS One. 2010 Aug 27;5(8):e12459. doi: 10.1371/journal.pone.0012459.
10
Monitoring of bacterial sugars and hydroxy Fatty acids in dust from air conditioners by gas chromatography-mass spectrometry.采用气相色谱-质谱法监测空调空气中的细菌糖和羟基脂肪酸。
Appl Environ Microbiol. 1993 Dec;59(12):4354-60. doi: 10.1128/aem.59.12.4354-4360.1993.
本文引用的文献
1
Studies on the biosynthesis of cell-wall polysaccharide in mutant strains of Salmonella. I.沙门氏菌突变株中细胞壁多糖生物合成的研究。I.
Proc Natl Acad Sci U S A. 1962 Aug;48(8):1337-41. doi: 10.1073/pnas.48.8.1337.
2
THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中甘油和L-α-磷酸甘油异化作用的控制
J Biol Chem. 1964 Sep;239:3106-8.
3
ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中L-α-甘油磷酸的主动运输
J Biol Chem. 1964 Sep;239:3098-105.
4
5
STUDIES ON THE GLUCOSE-TRANSPORT SYSTEM IN ESCHERICHIA COLI WITH ALPHA-METHYLGLUCOSIDE AS SUBSTRATE.以α-甲基葡萄糖苷为底物对大肠杆菌葡萄糖转运系统的研究。
Biochim Biophys Acta. 1963 Nov 15;78:505-15. doi: 10.1016/0006-3002(63)90912-0.
6
STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM.革兰氏阴性菌细胞壁的研究。I. 2-酮-3-脱氧辛酸在鼠伤寒沙门氏菌脂多糖中作用的证据。
Proc Natl Acad Sci U S A. 1963 Sep;50(3):499-506. doi: 10.1073/pnas.50.3.499.
7
Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella.细菌脂多糖的生物合成。I. 半乳糖在沙门氏菌突变株中的酶促掺入。
Proc Natl Acad Sci U S A. 1962 Oct 15;48(10):1831-8. doi: 10.1073/pnas.48.10.1831.
8
Guanosine diphosphate D-glycero-D-manno-heptose.
J Biol Chem. 1962 Feb;237:497-9.
9
A new method for the extraction of R lipopolysaccharides.一种提取R脂多糖的新方法。
Eur J Biochem. 1969 Jun;9(2):245-9. doi: 10.1111/j.1432-1033.1969.tb00601.x.
10
A hexose-phosphate transport system in Escherichia coli.大肠杆菌中的一种己糖磷酸转运系统。
Biochim Biophys Acta. 1966 Mar 28;117(1):231-40. doi: 10.1016/0304-4165(66)90170-x.
Zotero 插件